Sybr green real time polymerase chain reaction master mix

Caspase-3 inhibitor II (Z-DEVD-FMK) was purchased from Calbiochem. Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) was purchased from Roche. SYBR Green real-time polymerase chain reaction (PCR) master mix was purchased from TaKaRa (Shiga, Japan). The universal type SP kit and DAB kit were purchased from ZSGB-BIO. The Annexin V-FITC apoptosis detection kit and the cell cycle and apoptosis detection kit were purchased from Beyotime Biotechnology. The plasmids (GV230 and GV230-CDH1) were designed and synthesized by Shanghai Genechem Co., Ltd. The monoclonal antibody directed against the E-cadherin cytoplasmic domain (clone 36) was purchased from Transduction Laboratories (Lexington, KY). The polyclonal antibodies directed against H. pylori were purchased from Abcam (ab187301). The polyclonal antibodies directed against pro-caspase-3, caspase-3 (active), Bcl-2, and Bax were purchased from Sangon Biotech. The monoclonal antibody directed against GAPDH was purchased from Beyotime Biotechnology. HRP-anti-mouse antibody and HRP-anti-rabbit antibody were purchased from Boster Biological Technology. Cy3-labeled donkey anti-mouse antibody was purchased from Sangon Biotech.

The HIF-1α antibody was purchased from BD Biosciences (Palo Alto, CA). HK2, PDK1, LDHA, GLUT1 and LC3B antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The MT-CO1 antibody was purchased from Abcam (Cambridge, UK). Voltage-dependent anion channel (VDAC1), BCL2 interacting protein 3 (BNIP3) and β-tubulin were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Puromycin was purchased from Sigma-Aldrich Co. (Saint Louis, MO, USA). The SYBR green real-time polymerase chain reaction (PCR) master mix was purchased from Takara Bio Inc. (Kusatsu, Shiga, Japan).