Tumor tissues were digested with Hank’s balanced salt solution (HBSS; Life Technologies) containing 5 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES), 2% fetal bovine serum (FBS), 1 mg/mL collagenase (Wako Pure Chemical Industries), and 0.1% DNase I (Life Technologies), as described [16 (link)]. A total number of 106 cells were labeled with 0.5 μg of primary Ab (anti-nectin-4 goat polyclonal Antibody (R&D systems)) or isotype control in 100 μL of HBSS (Life Technologies) containing 2% FBS and HEPES, and then with an Alexa-Fluor-488-conjugated anti-goat IgG Ab (Life Technologies) diluted 1:500. To exclude dead cells, 7-AAD (Beckman Coulter) was used in the analysis. The cells were analyzed with a BD FACS Verse flow cytometer (BD Co.), and the data were processed using the Flow Jo FACS analysis software ver. 9.5.3.
Anti nectin 4 goat polyclonal antibody
Manufactured by R&D Systems
Sourced in United States
The Anti-nectin-4 goat polyclonal Antibody is a laboratory reagent used for the detection and quantification of nectin-4 in various experimental settings. It is a polyclonal antibody produced in goats and is designed for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase, by Fujifilm (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Thermo Fisher Scientific (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
7 aad, by Beckman Coulter (1 mentions)
Control goat igg, by R&D Systems (1 mentions)
Anti human slam mab clone 7d4, by BioLegend (1 mentions)
Facsverse, by BD (1 mentions)
2 protocols using anti nectin 4 goat polyclonal antibody
1
Tumor Cell Immunophenotyping by Flow Cytometry
2
Flow Cytometric Analysis of Nectin-4, SLAM, and CD46
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression of nectin‐4, SLAM, and CD46 was analyzed by flow cytometry according to previously reported methods.6 , 14 The following antibodies were used: anti‐human SLAM mAb (clone 7D4; Biolegend, San Diego, CA, USA); anti‐human CD46 mAb, anti‐nectin‐4 goat polyclonal antibody, mouse control IgG1, and goat control IgG (all from R&D Systems, Minneapolis, MN, USA); and Alexa 488‐conjugated anti‐mouse or anti‐goat antibody (Invitrogen). Cells were analyzed on a BD FACSVerse (BD Biosciences, San Diego, CA, USA). To compare the expression level of nectin‐4, the mean fluorescence intensity of cells stained with anti‐nectin‐4 antibody was calculated using FlowJo software version 9.7.5 (Tree Star, San Carlos, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!