Briefly, PC cells were plated in 24-well culture plates overnight, fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.2% Triton X-100 for 10 min, incubated with 5% BSA for 2 h and then stained for the primary antibodies: monoclonal anti-CRT antibody (Abcam, Cambridge, UK), CRT/pEGFR-Tyr1173 (pEGFR1173, Abcam), CRT/Fibronectin (Proteintech, Chicago, IL, USA), CRT/Integrinβ1 (Abcam) and CRT/ E-cadherin (E-cad, Abcam) in 4 °C overnight. The secondary antibodies were conjugated with green FITC (Proteintech) and red TRIC (Proteintech). Cells were then co-stained with Hoechst or DAPI (Sigma, St. Louis, MO, USA) for nuclei visualization and finally viewed by fluorescence microscope (Nikon Microphot-FX, Tokyo, Japan) and confocal fluorescence microscopy (Leica Tcs Sp5 II, Leica, Heidelberg, Germany).
Green fitc
Manufactured by Proteintech
The Green FITC is a fluorescent dye that emits green light when excited by a light source. It is commonly used in various laboratory applications to visualize and detect proteins, cells, and other biological molecules. The core function of the Green FITC is to provide a fluorescent label for these targets, enabling researchers to observe and analyze them using fluorescence microscopy or other fluorescence-based techniques.
Automatically generated - may contain errors
2 protocols using green fitc
1
Immunostaining of Cellular Protein Interactions
2
Immunofluorescence Staining of CRC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, we fixed CRC cells plated in 24-well culture plates using 4% paraformaldehyde at room temperature for 30min. The samples were then permeabilized in 1% Triton X-100 and blocked in 5% BSA for 2 h and then incubated with primary antibody [rabbit anti-GATA6 at 1:100 dilution (CST, USA) and mouse anti-β-catenin at 1:100 dilution (Proteintech, USA)] at 4 °C overnight. The secondary antibodies were then incubated with green FITC (Proteintech) and red TRIC (Proteintech). We finally co-stained the cells using DAPI (Sigma, St. Louis, MO, USA) for nuclei visualization and used a fluorescence microscope (Nikon Microphot-FX, Tokyo, Japan) for visualization.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!