Cd38 pe cy7 clone hit2

Manufactured by BioLegend

CD38-PE/Cy7 (clone HIT2) is a fluorescently-labeled monoclonal antibody that binds to the CD38 antigen. CD38 is a transmembrane glycoprotein expressed on various cell types, including lymphocytes, plasma cells, and some hematopoietic stem cells.

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2 protocols using cd38 pe cy7 clone hit2

Multiparametric Flow Cytometry Analysis

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Single-cell suspensions from the BM and peripheral blood were first incubated with human BD Fc block (BD Biosciences) to block Fcγ receptor and then stained with the following anti-human Abs: CD19-APC (clone HIB19, BioLegend), CD34-PE (clone 563, BD Biosciences), CD20-FITC (clone 2H7, BioLegend), CD27-APC/Cyanine7 (clone O323, BioLegend), IgD-PE (clone IA6-2, BD Biosciences), IgM-FITC (clone G20-127, BD Biosciences), CD43-PE (clone CD43-10G7, BioLegend), CD38-PE/Cy7 (clone HIT2, BioLegend), CD38-Percp/Cy5.5 (clone HIT2, BD Biosciences), CD24-PE (clone ML5, BD Biosciences), CD27-V450 (clone M-T271, BD Biosciences), IgD-BV510 (clone IA6-2, BD Biosciences), IgG-PE/Cy7 (clone G18-145, BD Biosciences), Ki-67–PE (clone Ki-67, BioLegend), CD80-FITC (clone L307.4, BD Biosciences), CD86-PE (clone IT2.2, BD Biosciences), HLADR-PE (clone G46-6, BD Biosciences) and CD69-PE/Cy7 (clone FN50, BD Biosciences). 7-AAD Viability Staining Solution (eBioscience, Thermo Fisher Scientific) was used for live versus dead cell discrimination. Samples were analyzed with a FACSVerse flow cytometer (BD Biosciences) using the FACSuite software. Data analysis was performed with FlowJo 10 software (Treestar).

Min Q., Meng X., Zhou Q., Wang Y., Li Y., Lai N., Xiong E., Wang W., Yasuda S., Yu M., Zhang H., Sun J., Wang X, & Wang J.Y. (2021). RAG1 splicing mutation causes enhanced B cell differentiation and autoantibody production. JCI Insight, 6(19), e148887.

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Multicolor Flow Cytometry Staining of B-cells

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Tonsillar single cell suspensions or B-lymphoma cell lines were stained with antibodies on ice for 30 min before analysis on a FACSCanto II (BD). The following antibodies from BioLegend were used: CD20-PacBlue (clone 2H7), CD3-BV570 (clone UCHT1), CD38-PECy7 (clone HIT2), CD79A-PECy7 (clone HM47) and IgM-PacBlue (clone MHM-88). The following antibodies from BD were used: CD27-APC (clone M-T271), CD27-BV605 and -FITC (clone L128), CD3-V500 and -Ax700 (clone UCHT1), CD79B-PerCPCy5.5 and -APC (clone SN8/3A2–2E7), IgD-PerCPCy5.5 (clone IA6–2) and IgG-APC-H7 (clone G18–145). In addition, we used CD3-PE (clone UCHT1) and poly rabbit IgD (cat. No. R5112) from Dako, CD79A-PE (clone 706931) from R&D, poly goat IgA-PE (Cat no. 2052–09) from Southern Biotech, and poly goat IgG- and IgM-FITC (Cat. No. AHI1308 and AHI 1608) from Invitrogen.

Huse K., Bai B., Hilden V.I., Bollum L.K., Våtsveen T.K., Munthe L.A., Smeland E.B., Irish J.M., Wälchli S, & Myklebust J.H. (2022). Mechanism of CD79A and CD79B support for IgM+ B cell fitness through BCR surface expression. Journal of immunology (Baltimore, Md. : 1950), 209(10), 2042-2053.

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