Bronchoalveolar lavage (BAL) was collected in Nfib hemizygous and Wt mice following 66 h of exposure to O2 or room air (N = 4 in each group). The trachea was cannulated and the airways were instilled with normal saline (1 mL × 3). Approximately 0.8 mL of saline was recovered per wash. The lavage fluid was centrifuged at 150 g for 10 min at 4°C. The supernatant of the first aliquot was stored at −80°C for cytokine assays. The pellet collected from the centrifuged tube was used for the cell counts. Cell counts were performed on the Coulter Counter Multisizer 3. Differential count was performed by counting cells on cytospin preparations of the resuspended cell pellet stained with diff‐Quik stain (Fisher Scientific, Pittsburgh, PA).
Diff quik stain
Manufactured by Thermo Fisher Scientific
The Diff-Quik stain is a quick and easy-to-use staining kit designed for the differential staining of blood and other cellular samples. It consists of three solutions that are used to stain and fix the samples, enabling the visualization of cellular structures and nuclei under a microscope.
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Lab products found in correlation
2 protocols using diff quik stain
1
Bronchoalveolar Lavage in Nfib Mice
2
Fungal Infection in Knockout Mice
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