The iNeurons were fixed in 4% paraformaldehyde in PBS for 20 min at room temperature and then blocked and permeabilized with 0.25% Triton X-100 and 1% BSA in PBS at room temperature for 1hr. Alternatively, the iNeurons were fixed and permeabilized in cold methanol at −20°C for 20 min and blocked in 2% BSA for 1hr. Anti-MAP2 antibody (Cell Signaling) and anti-β3 tubulin (TUBB3) antibody (Cell Signaling) were diluted at 1:200 in PBS with 0.05% Triton X-100 and 0.1% BSA and applied overnight at 4°C. Secondary antibodies (Thermo Fisher Scientific were diluted at 1:1000 in PBS with 0.05% Triton X-100 and 0.1% BSA) and applied for 1 hr at room temperature. Vectashield mounting medium for fluorescence with DAPI (Vector Laboratories) was used for nuclear counterstain. TUBB3+ cells in 3 fields were counted for each sample.
Anti β3 tubulin tubb3 antibody
Manufactured by Cell Signaling Technology
The Anti-β3 tubulin (TUBB3) antibody is a reagent used for the detection and analysis of the beta-3 isoform of tubulin, a key structural protein found in microtubules. This antibody can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and quantify the expression of TUBB3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti map2 antibody, by Cell Signaling Technology (2 mentions)
Vectashield mounting medium for fluorescence with dapi, by Vector Laboratories (1 mentions)
Vectashield mounting medium for fluorescence, by Vector Laboratories (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
2 protocols using anti β3 tubulin tubb3 antibody
1
Immunofluorescence Labeling of Neuronal Markers
2
Immunocytochemical Analysis of iNeurons
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The iNeurons were fixed in 4% paraformaldehyde in PBS for 20 min at room temperature and then blocked and permeabilized with 0.25% Triton X-100 and 1% BSA in PBS at room temperature for 1hr. Alternatively, the iNeurons were fixed and permeabilized in cold methanol at −20°C for 20 min and blocked in 2% BSA for 1hr. Anti-MAP2 antibody (Cell Signaling) and anti-β3 tubulin (TUBB3) antibody (Cell Signaling) were diluted at 1:200 in PBS with 0.05% Triton X-100 and 0.1% BSA and applied overnight at 4° C. Secondary antibodies (Thermo Fisher Scientific were diluted at 1:1000 in PBS with 0.05% Triton X-100 and 0.1% BSA) and applied for 1 hr at room temperature. Vectashield mounting medium for fluorescence with DAPI (Vector Laboratories) was used for nuclear counterstain. TUBB3+ cells in 3 fields were counted for each sample.
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