Donkey anti goat igg h l alexa fluor 488 568

Manufactured by Thermo Fisher Scientific

Sourced in United States

Donkey anti-goat IgG (H + L) Alexa Fluor (AF) 488/568 is a secondary antibody conjugate used in immunofluorescence and other fluorescence-based applications. The antibody is raised in donkeys and specifically binds to the heavy and light chains of goat immunoglobulin G (IgG). The antibody is labeled with Alexa Fluor 488 or Alexa Fluor 568 dyes, which emit green or orange-red fluorescence, respectively, upon excitation.

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Donkey anti-Goat IgG (H+L) Alexa Fluor 568 Alexa Fluor 488 donkey anti-goat IgG (H+L) Alexa Fluor 568 donkey anti-goat IgG Alexa Fluor 488 donkey anti-goat IgG Alexa Fluor 568 donkey anti-goat Alexa fluor 568 anti-goat IgG Alexa Fluor 488 donkey anti-goat Alexa 488 donkey-anti-goat IgG Alexa Fluor 488 anti-goat IgG Alexa Fluor IgG H + L

2 protocols using donkey anti goat igg h l alexa fluor 488 568

Immunohistochemical Profiling of Complement System

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Primary antibodies used for assays: Goat anti-rat C3 IgG (MP Biomedicals, Santa Ana, CA, United States), Chicken anti-C3/C3a (Abcam, Cambridge, MA, United States), Rabbit anti-rat C9 (generously provided by Professor Paul Morgan, Cardiff, United Kingdom), goat anti-human C1q (Complement Technology, Inc., Tyler, TX, United States), mouse anti-NeuN (EMD Millipore, Temecula, CA, United States), chicken anti-NeuN (EMD Millipore), mouse anti-GFAP (Sigma-Aldrich, St. Louis, MO, United States), chicken anti-GFAP (Abcam), goat anti-Iba1 (Abcam), rabbit anti-C3aR (Bioss Inc., Woburn, MA, United States), Mouse anti-C5aR (Hycult Biotech., Plymouth Meeting, PA, United States). Secondary antibodies included goat anti-mouse horseradish peroxidase (HRP) (Sigma-Aldrich), goat anti-chicken IgY HRP (Genway Biotech, San Diego, CA, United States), goat anti-rabbit HRP (Sigma-Aldrich), rabbit anti-goat HRP (Thermo Fisher Scientific, Grand Island, NY, United States), biotinylated goat anti-mouse IgG (H + L), biotinylated goat anti-chicken IgG (H + L) (Vector Laboratories, Burlingame, CA, United States), biotinylated goat anti-rabbit IgG (H + L) (Bioss Inc), donkey anti-goat IgG (H + L) Alexa Fluor (AF) 488/568, goat anti-chicken IgG (H + L) AF 488, goat anti-mouse IgG (H + L) AF 568, and goat anti-rabbit IgG (H + L) AF 405/488 (Life Technologies, Grand Island, NY, United States).

Shah T.A., Pallera H.K., Kaszowski C.L., Bass W.T, & Lattanzio F.A. (2021). Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy. Frontiers in Neuroscience, 15, 616734.

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Comprehensive Immunohistochemistry Panel

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Primary antibodies used for assays: Goat anti-rat C3 IgG (MP Biomedicals, Santa Ana, CA), Chicken anti-C3/C3a (Abcam, Cambridge, MA), Rabbit anti-rat C9 (generously provided by Professor Paul Morgan, Cardiff, UK), Mouse anti-C3aR (Thermo Fisher Scientific, Rockford, IL), Rabbit anti-C3aR (Bioss, Woburn, MA), Mouse anti-C5aR (Hycult Biotech., Plymouth Meeting, PA), goat anti-human C1q (Complement Technology, Tyler, Texas) mouse anti-NeuN (EMD Millipore, Temecula, CA), chicken anti-NeuN (EMD Millipore), mouse anti-GFAP (Sigma-Aldrich, St. Louis, MO), chicken anti-GFAP (Abcam), goat anti-Iba1 (Abcam) and mouse anti-GAPDH (Abcam). Secondary antibodies included goat antimouse horseradish peroxidase (HRP) (Sigma-Aldrich), goat antichicken IgY HRP (Genway Biotech, San Diego, CA), goat antirabbit HRP (Sigma-Aldrich), rabbit antigoat HRP (Thermo Fisher Scientific, Grand Island, NY), biotinylated goat antimouse IgG (H+L), biotinylated goat antichicken IgG (H+L) (Vector Laboratories, Burlingame, CA), Biotinylated goat antirabbit IgG (H+L) (Bioss), donkey antigoat IgG (H+L) Alexa Fluor (AF) 488/568, goat antichicken IgG (H+L) AF 488, goat antimouse IgG (H+L) AF 568, goat antirabbit IgG (H+L) AF 405/488 (Life Technologies, Grand Island, NY).

Shah T.A., Nejad J.E., Pallera H.K., Lattanzio F.A., Farhat R., Kumar P.S., Hair P.S., Bass W.T, & Krishna N.K. (2017). Therapeutic hypothermia modulates complement factor C3a and C5a levels in a rat model of hypoxic ischemic encephalopathy. Pediatric research, 81(4).

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