HA (Mw=20 kDa) was purchased from Lifecore Biomedical (Minnesota, USA). EGCG was purchased from DSM Nutritional Products Ltd (Heerlen, the Netherlands). Sorafenib tosylate was obtained from AbMole BioScience (Houston, USA). Amicon Ultra-15 centrifugal filters were purchased from Merck Millipore Corporation (Darmstadt, Germany). MesenCult-ACF and MethoCult H4434 Classic media were obtained from Stemcell Technologies (Vancouver, Canada). Dextran (Mw=70 kDa) and radioimmunoprecipitation assay (RIPA) buffer was purchased from Sigma-Aldrich (Minnesota, USA). Anti-HARE monoclonal antibody (mAb, MBL International, clone #34 − 2), anti-CD44 mAb (Thermo Fisher, clone Hermes-1) and control rat IgG (Thermo Fisher) were used as received. The following primary antibodies used for Western blotting were rabbit polyclonal unless otherwise stated: phospho-S6 (Ser235/236) and S6 (GeneTex, USA); phospho-STAT5a (Tyr694), STAT5a (C-term), phospho-ERK1/2 (Thr202/Tyr204) and ERK1/2 (Abcepta, USA); β-actin (mouse monoclonal, Proteintech, USA). Secondary antibodies (anti-rabbit/anti-mouse IgG-horseradish peroxidase conjugates) and CellTiter-Glo cell viability assay reagent were purchased from Promega (Madison, USA). RNeasy Mini kit (Qiagen, Germany), QuantiTect Reverse Transcription kit (Qiagen, Germany) and Micro BCA protein assay kit (Thermo Scientific, USA) were used per the manufacturer’s protocol.
Control rat igg
Manufactured by Thermo Fisher Scientific
Control rat IgG is a laboratory reagent used as a control for experiments involving rat immunoglobulin G (IgG). It provides a reference point to ensure the specificity and reliability of IgG-based assays and procedures.
Automatically generated - may contain errors
Lab products found in correlation
Mesencult acf, by STEMCELL (1 mentions)
Celltiter glo cell viability assay reagent, by Promega (1 mentions)
Radioimmunoprecipitation assay ripa buffer, by Merck Group (1 mentions)
Methocult h4434 classic, by STEMCELL (1 mentions)
Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Quantitect reverse transcription kit, by Qiagen (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
β actin, by Proteintech (1 mentions)
Amicon ultra 15 centrifugal filter, by Merck Group (1 mentions)
Keyhole limpet hemocyanin klh, by Biosearch Technologies (1 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
2 protocols using control rat igg
1
Hyaluronic Acid Biomedical Protocols
2
Optimizing Dendritic Cell and Splenocyte Culture Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cell culture medium for DCs and splenocytes was RPMI 1640 (Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal calf serum (FCS; Gibco-BRL) and 100 U/mL streptomycin-penicillin. Granulocyte-macrophage colony stimulating factor (GM-CSF; Lot no. 050755-1), IL-4 (Lot no.1106CY49), and IL-2 (Lot no.0608108) were obtained from Peprotech (Rocky Hill, NJ, USA). PC-keyhole limpet hemocyanin (PC-KLH) and keyhole limpet hemocyanin (KLH) were obtained from Biosearch Technologies (Novato, CA, USA). The purified anti-CD4 antibody (Ab; clone RM4-5) was from BD Systems (Franklin, NJ, USA). Fluorescein isothiocyanate (FITC)-conjugated anti-CD11c (Clone N418), phycoerythrin (PE)-conjugated anti-CD86 (Clone PO3.1), anti-major histocompatibility complex Class II (MHC-II)-APC (Clone HIS19), anti-CD40-PE (Clone 5C3), anti-CD45-FITC (Clone HI30), anti-CD19-APC (Clone HIB19), anti-CD4-FITC (Clone GK1.5), anti-CD25-APC (Clone PC61.5), anti-Foxp3-PE (Clone FJK-165), anti-interferon (IFN)-γ-PE (Clone XMG1.2), anti-IL-17A-PE (Clone eBio17B7), anti-Foxp3-biotin (Clone FJK-16 s), and anti-CD11c-biotin (Clone N418) Abs were obtained from eBioscience (San Diego, CA, USA). Anti-CD68 Ab was obtained from Millipore and anti-smooth muscle actin (SMA) Ab (Lot no. BM0002) was obtained from Boster (Wuhan, China). Control rat IgG was obtained from ThermoFisher Scientific (Rockford, IL, USA).
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