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2 protocols using anti transferrin receptor

1

Comprehensive Protein Immunodetection Protocol

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The following primary antibodies were used: Anti-Arfgef1 N-terminal epitope (ThermoFisher Scientific, PA5-54894; Santa Cruz, sc-376790; sc-50391), anti-Arfgef1 C-terminal epitope (Bethyl, A300-998A), anti-Arfgef2 C-terminal epitope (Sigma-Aldrich, MABS1246), Anti-GABAA Receptor with epitope for extracellular domain of the β2,3 subunits (Millipore Sigma, MAB341), anti-synaptophysin (ThermoFisher Scientific, PA1-1043), anti-lamp1 (DSHB, 1D4B), anti-eea1 (R&D Systems, AF8047), anti-transferrin receptor (Abcam, ab84036), anti-Rab11 (ThermoFisher Scientific, 71-5300), anti-Arf6 (ThermoFisher Scientific, PA1-093), anti-map2 (Millipore Sigma, Ab5622), anti-ankG (Santa Cruz, sc-28561), anti-ß-tubulin (Proteintech, 10094-1-AP and 66240-1-Ig), anti-GAPDH (Proteintech, 60004-1-Ig), anti-lamin B1 (Proteintech, 66095-1-lg). The following secondary antibodies were used: Goat anti-mouse HRP-conjugated IgG (Proteintech, SA00001-1), goat anti-rabbit HRP-conjugated IgG (Proteintech, SA00001-2), Goat anti-mouse Alexa Fluor 488-conjugated IgG (ThermoFisher Scientific, A11017), goat anti-rabbit Alexa Fluor 594-conjugated IgG (ThermoFisher Scientific, A11072), goat anti-rat Alexa Fluor 594-conjugated IgG (ThermoFisher Scientific, A11007), donkey anti-sheep NL557-conjugated IgG (R&D Systems, NL010).
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2

Antibody-based Protein Analysis Protocol

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The following antibodies were used: anti-β-actin (Sigma), anti-c-myc (Roche), anti-DMT1 (Abnova), anti-ferroportin (Novus Biologicals, Littleton, CO), anti-GAPDH (Abcam), anti-Parkin clone PRK8 (Covance, Princeton, NJ), anti-α-synuclein (BD Biosciences), anti-TH (Pel-Freez Biologicals, Rogers, AR), anti-transferrin receptor (Abcam) and HRP-conjugated anti-mouse and anti-rabbit (Sigma).
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