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Horseradish peroxidase conjugated anti mouse or anti rat antibodies

Manufactured by Southern Biotech
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Horseradish peroxidase-conjugated anti-mouse or anti-rat antibodies are laboratory reagents used for the detection and quantification of target proteins in various immunoassays. The horseradish peroxidase enzyme is conjugated to the antibody, allowing for the amplification and visualization of the target protein signal.

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Lab products found in correlation

Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) A5441, by Merck Group (2 mentions) β actin, by Merck Group (2 mentions)

Close spelling variants of this product

Horseradish peroxidase (23 citations) Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rat antibodies (2 citations)

2 protocols using horseradish peroxidase conjugated anti mouse or anti rat antibodies

1

Western Blot Analysis of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell extracts (15µg) were analyzed on 12% SDS-PAGE under reducing conditions, transferred to PVDF membranes and probed with antibodies against HA (3F10, 1:5000, Roche Applied Science) or β-actin (A5441, 1:5000, Sigma-Aldrich). Proteins of interest were detected with horseradish peroxidase-conjugated anti-mouse or anti rat antibodies (1:5000, SouthernBiotech) and visualized with the Pierce ECL Western blotting substrate (Thermo Scientific), according to the manufacturer’s protocol.
Legati A., Giovannini D., Nicolas G., López-Sánchez U., Quintáns B., Oliveira J., Sears R.L., Marisa Ramos E., Spiteri E., Sobrido M.J., Carracedo Á., Castro-Fernández C., Cubizolle S., Fogel B.L., Goizet C., Jen J.C., Kirdlarp S., Lang A.E., Miedzybrodzka Z., Mitarnun W., Paucar M., Paulson H., Pariente J., Richard A.C., Salins N.S., Simpson S.A., Striano P., Svenningsson P., Tison F., Unni V.K., Vanakker O., Wessels M.W., Wetchaphanphesat S., Yang M., Boller F., Campion D., Hannequin D., Sitbon M., Geschwind D.H., Battini J.L, & Coppola G. (2015). Mutations in XPR1 cause primary familial brain calcification associated with altered phosphate export. Nature genetics, 47(6), 579-581.
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2

Western Blot Analysis of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell extracts (15µg) were analyzed on 12% SDS-PAGE under reducing conditions, transferred to PVDF membranes and probed with antibodies against HA (3F10, 1:5000, Roche Applied Science) or β-actin (A5441, 1:5000, Sigma-Aldrich). Proteins of interest were detected with horseradish peroxidase-conjugated anti-mouse or anti rat antibodies (1:5000, SouthernBiotech) and visualized with the Pierce ECL Western blotting substrate (Thermo Scientific), according to the manufacturer’s protocol.
Legati A., Giovannini D., Nicolas G., López-Sánchez U., Quintáns B., Oliveira J., Sears R.L., Marisa Ramos E., Spiteri E., Sobrido M.J., Carracedo Á., Castro-Fernández C., Cubizolle S., Fogel B.L., Goizet C., Jen J.C., Kirdlarp S., Lang A.E., Miedzybrodzka Z., Mitarnun W., Paucar M., Paulson H., Pariente J., Richard A.C., Salins N.S., Simpson S.A., Striano P., Svenningsson P., Tison F., Unni V.K., Vanakker O., Wessels M.W., Wetchaphanphesat S., Yang M., Boller F., Campion D., Hannequin D., Sitbon M., Geschwind D.H., Battini J.L, & Coppola G. (2015). Mutations in XPR1 cause primary familial brain calcification associated with altered phosphate export. Nature genetics, 47(6), 579-581.
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