Standards and samples were analysed using gas chromatography flame ionisation detection (GC-FID) using a Varian 3800 GC system, fitted with a DB-FFAP column (30 mL × 0.32 mm ID × 0.25 μm df; Agilent) and a flame ionisation detector. Samples and standards were loaded (0.2 μL splitless injection) with a CP-8400 autosampler (Agilent). Helium was employed as the carrier gas at a flow rate of 1.3 mL/min. The initial GC oven temperature was set at 50 °C, and was maintained for 0.5 min, raised to 140 °C at 10 °C/min and held for 0.5 min, before being increased to 240 °C at 20 °C/min, and held for 5.0 min (total run time 20 min). The temperatures of the detector and the injection port were set at 300 °C and 240 °C, respectively. Peaks were integrated using Varian Star Chromatography Workstation version 6.0 software. Blank water vials were analysed between each sample duplicate to check for any potential carryover.
3800 gc system
Manufactured by Agilent Technologies
The Agilent 3800 GC system is a gas chromatograph designed for the separation, identification, and quantification of complex mixtures of volatile and semi-volatile organic compounds. It features a compact design, intuitive software, and high-performance components to deliver reliable and accurate results.
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3 protocols using 3800 gc system
1
GC-FID Analysis of Organic Compounds
2
GC-MS Analysis of Chemical Compounds
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The GC–MS instrument used for the analysis was Varian 3800 GC system equipped with a triple quadrupole mass spectrometer. The column used was (30 m × 0.25 mm, 0.25 μm). The carrier gas used was helium operated at a constant flow of 1 mL/min. The acquisition was in full scan mode in the m/z range 40–450 and identification was by exploiting the NIST and SWGDRUG4 library. The oven program was: initial isotherm 60 °C for 3 min, 40 °C/min to 180 °C and 10 °C/min to 300 °C, final isotherm 300 °C for 10 min. Injector and transfer line temperatures were 275 °C and 280 °C, respectively. The injection volume was 1 μl in splitless mode.
3
GC-FID Analysis of Illicit Drugs
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The analyses were carried out with Varian 3800 GC system equipped with an FID detector. The columns used were: 30 m × 0.25 mm, 0.25 μm for cocaine, heroin and benzodiazepines. 25 m × 0.25 mm, 0.25 μm was used for methamphetamine. The temperature programs were: i) for cocaine and heroin and benzodiazepine, starting temperature was 210 °C (hold time: 1 min), raised to 280 °C (increase rate: 25 °C/min; hold time: 1 min) and then 300 °C (increase rate: 25 °C/min; hold time: 1 min); ii) for methamphetamine, starting temperature was 100 °C (hold time: 1 min), raised to 125 °C (increase rate: 25 °C/min) and then 180 °C (increase rate: 15 °C/min). The carrier gas used was hydrogen set at the constant flow of 1 mL/min. The injection volume was 2 μl.
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