Anti α-tubulin, doxycycline, crystal violet and cantharidin were purchased from Sigma. Anti γ-tubulin and phospho Plk1 antibodies were purchased from Biolegend. Antibodies against pan phospho aurora kinase, HA, phospho histone3 (Ser 10) and cyclin B were purchased from Cell Signaling Technology. Anti-BubR1 antibody was purchased from BD Biosciences, and centromere autoantibodies were purchased from Immunovision. Secondary FIT-C and TRIT-C antibodies were obtained from Jackson Immunoresearch Laboratories. Secondary Alexa 488 and Alexa 568 were obtained from BD Biosciences. Aphidicolin, hydroxyurea, etoposide and okadaic acid were obtained from Calbiochem. pTREX plasmid was a gift from Novartis Pharmaceuticals. PyST was cloned in the pTREX plasmid using standard molecular biology techniques. Dominant negative p53 (pWZL-p53DD) was a gift from Dr. Jean Zhao. ST-HA-FLAG was also cloned in pBABE-puro-IRES-GFP (obtained from Addgene # 14430). PyST-Cdt1 and Geminin degron constructs were cloned by swapping PyST for SV40ST in the MSCV-puro-SV40-Cdt1/Geminin plasmids generated in Dr. David Livingston's lab. pRXTN50 (link) plasmid was a gift from Dr. James Iglehart.
Secondary alexa 488
Manufactured by BD
The Secondary Alexa 488 is a fluorescent dye used in various immunoassays and cell-based applications. It is designed to bind to primary antibodies, allowing for the detection and visualization of target proteins or cells.
Automatically generated - may contain errors
Lab products found in correlation
Cantharidin, by Merck Group (2 mentions)
Doxycycline, by Merck Group (2 mentions)
Crystal violet, by Merck Group (2 mentions)
Cyclin b, by Cell Signaling Technology (2 mentions)
Anti γ tubulin, by BioLegend (2 mentions)
Phospho plk1 antibodies, by BioLegend (2 mentions)
Anti bubr1 antibody, by BD (2 mentions)
Secondary fit c and trit c antibodies, by Jackson ImmunoResearch (2 mentions)
Pbabe puro ires gfp, by Addgene (2 mentions)
Anti α tubulin, by Merck Group (2 mentions)
2 protocols using secondary alexa 488
1
Molecular Tools for Cell Cycle Research
2
Molecular Tools for Cell Cycle Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti α-tubulin, doxycycline, crystal violet and cantharidin were purchased from Sigma. Anti γ-tubulin and phospho Plk1 antibodies were purchased from Biolegend. Antibodies against pan phospho aurora kinase, HA, phospho histone3 (Ser 10) and cyclin B were purchased from Cell Signaling Technology. Anti-BubR1 antibody was purchased from BD Biosciences, and centromere autoantibodies were purchased from Immunovision. Secondary FIT-C and TRIT-C antibodies were obtained from Jackson Immunoresearch Laboratories. Secondary Alexa 488 and Alexa 568 were obtained from BD Biosciences. Aphidicolin, hydroxyurea, etoposide and okadaic acid were obtained from Calbiochem. pTREX plasmid was a gift from Novartis Pharmaceuticals. PyST was cloned in the pTREX plasmid using standard molecular biology techniques. Dominant negative p53 (pWZL-p53DD) was a gift from Dr. Jean Zhao. ST-HA-FLAG was also cloned in pBABE-puro-IRES-GFP (obtained from Addgene # 14430). PyST-Cdt1 and Geminin degron constructs were cloned by swapping PyST for SV40ST in the MSCV-puro-SV40-Cdt1/Geminin plasmids generated in Dr. David Livingston's lab. pRXTN50 (link) plasmid was a gift from Dr. James Iglehart.
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