Deuterated chloroform

Ethyl acetate and n-hexane were purchased from VWR International (Radnor, PA, USA). Methanol and dichloromethane were obtained from Fisher Scientific (Loughborough, UK) and dimethyl sulfoxide and urea were supplied by Panreac Quimica SAU (Castellar del Vallés, Barcelona, Spain). Deuterated chloroform for spectroscopic experiments was obtained from VWR Chemicals Prolabo^® (Leuven, Belgium). For the cytotoxicity bioassays, Dulbecco’s Modified Eagle’s Medium (DMEM) was supplied by Lonza (Verviers, Belgium), premixed phosphate buffer saline solution (PBS, 10×) was supplied by Roche (Steinheim, Germany), fetal bovine serum, penicillin/streptomycin, l-glutamine, sodium pyruvate, trypsin and minimum essential medium non-essential amino acids (MEM NEAA), RPMI 1640 medium were purchased from Gibco (Paisley, UK). α-Cyclodextrin (α-CD) was obtained from Tokyo Chemical Industry (TCI Europe, Zwijndrecht, Belgium). Water (type I) was obtained from an Ultramatic system from Wasserlab. Lipase from porcine pancreas (L3126; enzymatic activity of 100–500 units), mucin from porcine stomach type II (M2378), pancreatin from porcine pancreas (P7545; 8 × U.S.P. specifications) and pepsin from porcine stomach mucosa (P7000; enzymatic activity of 500 units/mg solid) were obtained from Sigma Aldrich (Steinheim, Germany).

Poly(ethylene glycol) (PEG, 2 kDa (M_n = 1917) and 10 kDa (M_n = 12 157)), acryloyl chloride, triethylamine, potassium bicarbonate, sodium sulfate and Irgacure 2959 were purchased from Sigma-Aldrich (St Louis, MO). Dichloromethane and deuterated chloroform with 0.03 vol% TMS were purchased from VWR Chemicals (Radnor, PA). Diethyl ether and Dulbecco's phosphate buffered saline were purchased from Fisher Scientific (Hampton, NH). SecureSeal Imaging Spacers were purchased from Grace Bio-Labs (Bend, OR). All reagents were used as received unless specified otherwise.