Cell invasion and migration were conducted using 8‐µm transwell inserts (Costar) coated with or without 60 µL of Matrigel (BD Biosciences), which were placed into each well of a 24‐well plate. Forty‐eight hours after transfection, SGC‐7901 and BGC‐823 cells (density 2 × 104) in 200 µL of serum‐free medium were transferred to the upper chamber following the methods previously described.26 Cells were stained with crystal violet and counted by direct microscopic visualization.
8 m transwell inserts
Manufactured by Corning
Sourced in United States
The 8-μm transwell inserts are a type of lab equipment designed for cell culture applications. They feature a porous membrane with 8-micrometer pores that allow for the movement of cells and media between the upper and lower chambers of the transwell system.
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Lab products found in correlation
4 protocols using 8 m transwell inserts
1
Transwell-Based Cell Invasion and Migration
2
HUVEC Migration Assay with ZK002
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HUVECs were seeded at a density of 5 × 104 cells per well onto 8 µm transwell inserts (Costar, Corning, NY, United States). The lower chamber was filled with 500 μL low serum cell culture medium only, or low serum cell culture medium containing VEGF (10 ng/mL). Cells were then treated with 0, 1, or 5 µM ZK002. After 4 h at 37°C, non-migrated cells were removed with cotton buds and migrated cells were fixed with 4% formaldehyde and stained for 10 min with crystal violet. The magnitude of HUVEC migration was evaluated by counting migrated cells in six random high-power (×100) microscope fields.
3
Transwell Migration Assay for VSMCs
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VSMCs were seeded at 5 × 104 per well onto 8-µm Transwell inserts (Corning Inc.; Corning, NY, USA). Lower chambers were filled with 500 μL of containing each CM and incubated for 48 h in a 5% CO2 atmosphere. Migration activities were evaluated using the WST assay as described above.
4
Cell Invasion Assay for Hormone Regulation
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Invasion assays were performed as described [40 (link)]. Briefly, 2.5 × 104 of parental and stably transfected TC11 cells were plated on 8-µm transwell inserts (Corning) coated with collagen I, and 5% CSS was added to the bottom chamber as a chemo-attractant. The parental cells were treated with ethanol Veh, 1 nM E2, or 10 μM ICI and the stably transfected TC11 cells were treated with ethanol Veh, 1 nM E2, or 1 μM ICI for 24 hours. Membranes from the transwells were stained with Wright-Giemsa, and positive staining was assessed in five random 10× microscopic fields from three independent experiments.
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