Chemicals, human IgG, and Protein A from Staphylococcus aureus were purchased for Sigma-Aldrich (St. Louis, MO), and HPLC-grade reagents were purchased from Fisher Scientific™ (Waltham, MA). Lys-C was purchased from FUJIFILM Wako Chemicals (Richmond, VA). HEK293 cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA). Yarra-SEC-4000 column (300 × 7.8 mm, pore size 500 Å, particle size 3 μm) was purchased from Phenomenex® (Torrance, CA). Empore™ Extraction Disks (3M C18) were purchased from VWR (Radnor, PA). iRT Kit was purchased from Biognosys Inc. (Boston, MA).
Yarra sec 4000 column
Manufactured by Phenomenex
The Yarra-SEC-4000 column is a size exclusion chromatography (SEC) column designed for the separation and analysis of macromolecules and proteins. The column features a high-performance porous silica-based stationary phase optimized for the separation of compounds with a wide range of molecular weights.
Automatically generated - may contain errors
Lab products found in correlation
Lys c, by Fujifilm (1 mentions)
Hplc grade reagents, by Thermo Fisher Scientific (1 mentions)
Protein a from staphylococcus aureus, by Merck Group (1 mentions)
Human igg, by Merck Group (1 mentions)
Hek293 cell line, by American Type Culture Collection (1 mentions)
Dawn heleos 8 mals detector, by Wyatt Technology (1 mentions)
Optilab t rex refractive index detector, by Wyatt Technology (1 mentions)
2 protocols using yarra sec 4000 column
1
Purification and Characterization of IgG
2
Molecular Weight Determination of Aggregates
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The molecular weight of the aggregates was determined using SEC in combination with multi-angle light scattering (MALS). SEC-MALS was performed using an Äkta Explorer 100 instrument (GE Healthcare) equipped with a Yarra SEC-4000 column (Phenomenex), an Optilab T-Rex refractive index (RI) detector (Wyatt) and a DAWN HELEOS 8+ MALS detector (Wyatt). The samples were analyzed at room temperature (RT) using 0.1 μm filtered PBS (pH 7.0).
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