All images except those of D. melanogaster were acquired using an inverted microscope (IX71; Olympus, Japan) equipped with a dark-field condenser and a piezo (P-725.xDD PIFOC; Physik Instrumente, Germany) to adjust the axial position of the objective. For MFI, the multi-channel imaging device (QV-2, Photometrics, USA) was installed in front of a high-speed camera (PCO Dimax, Germany). Different objectives were used: ×20 (UPLFLN, NA 0.5; Olympus, Japan), ×10 (UPlanSapo, NA 0.4; Olympus, Japan), ×4 (UPlanFLN, NA 0.13; Olympus, Japan). The total magnification could be increased with an additional ×1.6 magnification changer. Sperm, Amoeba proteus, and Hydra vulgaris were investigated in a custom-made observation chamber of 150 µm depth39 (link). Images of D. melanogaster were recorded with a stereomicroscope (SZX12; Olympus, Japan), equipped with a DF PLAPO 1X PF lens (NA 0.11; Olympus, Japan).
Df plapo 1x pf lens
Manufactured by Olympus
Sourced in Japan
The DF PLAPO 1X PF lens is a high-quality optical lens designed for use in various laboratory applications. It has a magnification of 1X and is part of the DF PLAPO series from Olympus. The lens is intended to provide clear and accurate imaging for researchers and scientists.
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2 protocols using df plapo 1x pf lens
1
Multi-Organism Imaging Protocols
2
Multimodal Imaging of Diverse Cellular Organisms
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All images except those of D. melanogaster were acquired using an inverted microscope (IX71; Olympus, Japan) equipped with a dark-field condenser and a piezo (P-725.xDD PIFOC; Physik Instrumente, Germany) to adjust the axial position of the objective. For MFI, the multi-channel imaging device (QV-2, Photometrics, USA) was installed in front of a high-speed camera (PCO Dimax, Germany). Different objectives were used: 20x (UPLFLN, NA 0.5; Olympus, Japan), 10x (UPlanSapo, NA 0.4; Olympus, Japan), 4x (UPlanFLN, NA 0.13; Olympus, Japan). The total magnification could be increased with an additional 1.6x magnification changer. Sperm, Amoeba proteus, and Hydra vulgaris were investigated in a custom-made observation chamber of 150 µm depth 39 . Images of D. melanogaster were recorded with a stereomicroscope (SZX12; Olympus, Japan), equipped with a DF PLAPO 1X PF lens (NA 0.11; Olympus, Japan).
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