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2 protocols using 1 p hydroxyphenyl imidazole

1

Synthesis and Analysis of Sulfur Compounds

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3-aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na2S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), S-nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-d-1-thiogalactopyranoside (IPTG) were obtained from Sigma-Aldrich. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red were obtained from Thermo Fisher Scientific. DEPMPO was obtained from Enzo. H2O2 was obtained from Roth. GSH and 5-aminolevulinic acid hydrochloride were obtained from Merck. Sodium disulfide (Na2S2), sodium tetrasulfide (Na2S4) and 3′,6′-di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS34SSG and GSSSSG was performed as described previously43 . CSSSC was synthesized as described below.
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2

Redox Signaling Compound Synthesis and Detection

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3-Aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na2S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7- (Diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3-MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), S- nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-D-1-thiogalactopyranoside (IPTG) were obtained from Sigma. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red was obtained from ThermoFisher. DEPMPO was obtained from Enzo. H2O2 was obtained from Roth. Glutathione (GSH) and 5- aminolevulinic acid hydrochloride was obtained from Merck. Sodium disulfide (Na2S2), sodium tetrasulfide (Na2S4), and 3',6'-Di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS34SSG and GSSSSG was performed as described previously 43 . CSSSC was synthesized as described below.
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