1400w dihydrochloride

Manufactured by Cayman Chemical

Sourced in United States

1400W dihydrochloride is a chemical compound used in laboratory settings. It serves as a core functional component in various research and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Fg 4592, by Cayman Chemical (2 mentions) N n 3 5 difluorophenacetyl l alanyl s phenylglycine t butyl ester dapt, by Merck Group (1 mentions) Noradrenaline, by Merck Group (1 mentions) L name, by Cayman Chemical (1 mentions) Noc 18, by Cayman Chemical (1 mentions)

3 protocols using 1400w dihydrochloride

Vascular Reactivity Evaluation Protocol

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Nor-adrenaline (NA) and N-[N-(3,5-Difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) were purchased from Sigma (St. Louis, Mo, USA) while 1400 W dihydrochloride was procured from Cayman (Ann Arbor, Michigan). All other used chemicals/reagents were of analytical grade. Nor-adrenaline was dissolved in 0.1 N hydrochloric acid and DAPT was dissolved in dimethyl sulfoxide (DMSO). All other chemicals were dissolved in distilled water. Concentration of the vehicle (DMSO) used had no significant effect of its own on vascular reactivity.

Singh V., Akash R., Chaudhary G., Singh R., Choudhury S., Shukla A., Prabhu S.N., Gangwar N, & Garg S.K. (2022). Sepsis downregulates aortic Notch signaling to produce vascular hyporeactivity in mice. Scientific Reports, 12, 2941.

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Modulation of CD8+ T cell function

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For each treatment, the same batch of cells was either incubated for 3 days with IL-2 supplemented T-cell media containing the experimental compound or DMSO vehicle control (< 0.1% of total well volume to avoid unspecific toxicity). The prolyl hydroxylase inhibitor FG-4592 (Cayman Chemicals) was used at 50 μM in wild-type mouse CD8⁺ T cells cultured in 1%O₂. The NOS2 inhibitor 1400W dihydrochloride (Cayman Chemicals) was used at 100 μM in mouse CD8⁺ T cells transduced with VC or NOS2^OE vectors following enrichment of Thy-1.1⁺ cells using MACS as described below. Human CD8⁺ T cells were treated with the NO donor compound NOC-18 (Cayman Chemicals) or with the panNOS inhibitor L-NAME (Cayman Chemicals) at concentrations ranging from 1 to 256 μM. T cells treated with the different compounds were then analyzed as detailed in figure legends.

Cunha P.P., Bargiela D., Minogue E., Krause L.C., Barbieri L., Brombach C., Gojkovic M., Marklund E., Pietsch S., Foskolou I., Branco C.M., Veliça P, & Johnson R.S. (2022). Infiltration of Tumors Is Regulated by T cell–Intrinsic Nitric Oxide Synthesis. Cancer Immunology Research, 11(3), 351-363.

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Modulating T-cell Metabolism and Function

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For each treatment, the same batch of cells was either incubated for 3 days with IL2 supplemented T-cell media containing the experimental compound or DMSO vehicle control (<0.1% of total well volume to avoid unspecific toxicity). The prolyl hydroxylase inhibitor FG-4592 (Cayman Chemicals) was used at 50 μmol/L in wild-type (WT) mouse CD8⁺ T cells cultured in 1% O₂. The NOS2 inhibitor 1400W dihydrochloride (Cayman Chemicals) was used at 100 μmol/L in mouse CD8⁺ T cells transduced with VC or NOS2^OE vectors following enrichment of Thy-1.1⁺ cells using MACS as described below. Human CD8⁺ T cells were treated with the NO donor compound 2,2′-(Hydroxynitrosohydrazino) bis-ethanamine (NOC-18; Cayman Chemicals) or with the panNOS inhibitor N(gamma)-nitro-L-arginine methyl ester (L-NAME; Cayman Chemicals) at concentrations ranging from 1 to 256 μmol/L. T cells treated with the different compounds were then analyzed as detailed in figure legends.

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