Log In Sign up
The largest database of trusted experimental protocols

α glut1

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

α-Glut1 is a primary antibody that recognizes the glucose transporter 1 (Glut1) protein. Glut1 is a transmembrane protein responsible for basal glucose uptake in various cell types. The α-Glut1 antibody can be used to detect and quantify Glut1 expression through techniques such as Western blotting, immunohistochemistry, and flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

α axin, by Cell Signaling Technology (2 mentions) α cd44, by Cell Signaling Technology (2 mentions) α hif 1a, by Cell Signaling Technology (2 mentions) α vegfa, by Abcam (2 mentions) α β actin, by Abcam (2 mentions) α β catenin, by Cell Signaling Technology (2 mentions) α c myc, by Cell Signaling Technology (2 mentions) α e cadherin, by Cell Signaling Technology (2 mentions) α cyclin d1, by Cell Signaling Technology (2 mentions) α histone h3, by Cell Signaling Technology (2 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Beyotime (2 mentions)

2 protocols using α glut1

1

Protein Expression Analysis in Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, α-cyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
Xie W., Zhao H., Wang F., Wang Y., He Y., Wang T., Zhang K., Yang H., Zhou Z., Shi H., Wang J, & Huang G. (2021). A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 40, 30.
+ Open protocol
+ Expand
2

Western Blot Analysis of EMT Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, αcyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
Xie W., Zhao H., Wang F., Wang Y., He Y., Wang T., Zhang K., Yang H., Zhou Z., Shi H., Wang J., & Huang G. (2020). A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!