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Recombinant trail

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Recombinant TRAIL is a protein produced using recombinant DNA technology. It functions as a ligand that can bind to death receptors on the cell surface, potentially inducing programmed cell death.

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Lab products found in correlation

Annexin 5 fitc, by BD (1 mentions) Trypsin, by Merck Group (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Accutase, by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) 1536 well plates, by Greiner (1 mentions)

Spelling variants of this product

Soluble recombinant human TRAIL (11 citations) Recombinant human TRAIL (5 citations)

2 protocols using recombinant trail

1

Apoptosis and Necrosis Quantification

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Cells were stimulated with the indicated genotoxic and proapoptotic agents, including bleomycin (Medac), hydrogen peroxide, thapsigargin, tunicamycin, brefeldin A (all from Sigma), as well as recombinant TRAIL and FasL (both from Enzo Life Sciences). After cell harvest using trypsin for differentiated cells and accutase (Millipore) for iPSCs, supernatants containing dead cells and the detached cells were combined. Subsequently, cells were stained with FITC-annexin-V and PI following the instructions of the manufacturer (BD Biosciences) and analyzed by flow cytometry. Cells with positive annexin-V but negative PI staining were considered apoptotic, whereas double-positive cells were considered necrotic.
Dannenmann B., Lehle S., Hildebrand D.G., Kübler A., Grondona P., Schmid V., Holzer K., Fröschl M., Essmann F., Rothfuss O, & Schulze-Osthoff K. (2015). High Glutathione and Glutathione Peroxidase-2 Levels Mediate Cell-Type-Specific DNA Damage Protection in Human Induced Pluripotent Stem Cells. Stem Cell Reports, 4(5), 886-898.
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2

High-Throughput TRAIL Cytotoxicity Assay

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H460-M cells were dispensed in two sets of 1536-well plates (Greiner
Bio-One) at 1000 cells/well. After overnight incubation, compounds were added at
multiple doses beginning at 46 μmol/L and diluted 3-fold with DMSO.
After 48 hours, each set of plates received 1 μL of PBS or recombinant
TRAIL (30 ng/mL final concentration, Enzo Life Sciences). Cell viability was
assessed at four hours post-TRAIL addition (PBS for vehicle set) using
CellTiter-Glo reagent (Promega) and luminescence (RLU) was quantified using a
ViewLux (PerkinElmer). A more detailed description of the qHTS is provided as
Supplemental Material and
Methods.
Hamilton D.H., Griner L.M., Keller J.M., Hu X., Southall N., Marugan J., David J.M., Ferrer M, & Palena C. (2016). Targeting estrogen receptor signaling with fulvestrant enhances immune and chemotherapy-mediated cytotoxicity of human lung cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(24), 6204-6216.
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