Fluorescent probe 2,7-dichloro uorescein diacetate (DCF-DA) and (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) were purchased from Sigma. RPMI 1640 was purchased from Bioidea Company and FBS was purchased from Gibco. Rabbit monoclonal anti-serums against procaspase-3, caspase-3cleaved, Bcl-2, caspase-8, and ß-actin, rabbit polyclonal anti-serum against Bax, and anti-mouse and antirabbit IgG HRP-linked antibodies were purchased from Cell signaling. Cadmium and minocycline were purchased from Tinab Shimi. Polyvinylidene uoride (PVDF) membrane was purchased from Bio-Rad.
Cell culture PC12 (pheochromocytoma cell line of rat) cells were obtained from the Pasteur Institute of Iran (Tehran). Cells were cultured in RPMI-1640 medium and incubated at 37˚C with 5% CO 2 . The medium contained 10% fetal bovine serum and 5% antibiotic mixture (100 U/mL penicillin and 100 µg/mL streptomycin) and was changed every 2-3 days.
Cell culture PC12 (pheochromocytoma cell line of rat) cells were obtained from the Pasteur Institute of Iran (Tehran). Cells were cultured in RPMI-1640 medium and incubated at 37˚C with 5% CO 2 . The medium contained 10% fetal bovine serum and 5% antibiotic mixture (100 U/mL penicillin and 100 µg/mL streptomycin) and was changed every 2-3 days.