Isopropyl β d thiogalactopyranoside

Manufactured by Euromedex

Sourced in France

Isopropyl β-D-thiogalactopyranoside is a laboratory chemical compound commonly used as an inducer in protein expression systems. It functions by activating the lac operon, a genetic regulatory system found in Escherichia coli and other bacterial species, leading to the production of recombinant proteins.

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Lab products found in correlation

Yeast extract, by Solabia (3 mentions) Lb medium, by Merck Group (3 mentions) Glycerol, by Euromedex (3 mentions) Ampicillin, by Eurobio Scientific (3 mentions) Bacto tryptone, by Solabia (3 mentions) Complete mini edta free protease inhibitor, by Roche (2 mentions) Hexafluoroisopropanol, by Merck Group (1 mentions) Sodium ascorbate, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Avantor (1 mentions) Methanol, by Avantor (1 mentions) N n n n n pentamethyl diethylenetriamine pmdeta, by Merck Group (1 mentions) Trizma, by Merck Group (1 mentions) Ethanol, by Avantor (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Cuso4 5h2o, by Avantor (1 mentions) Milli q biocel a10 purification unit, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Formic acid, by Merck Group (1 mentions)

4 protocols using isopropyl β d thiogalactopyranoside

Clickable Glycoconjugate Synthesis Protocol

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Bacto-tryptone and yeast extract were obtained from Biokar Diagnostics (FR). LB medium was purchased from Sigma-Aldrich (FR). Ampicillin was purchased from Eurobio (FR). Glycerol and isopropyl β-D-thiogalactopyranoside (IPTG) were purchased from Euromedex (FR).
Glycidyl propargyl ether was obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, FR).
Glacial acetic acid, Trizma® and Hexafluoroisopropanol (HFIP) were obtained from Sigma-Aldrich (FR). Deionized water (18 MΩ-cm) was obtained by using a Millipore Milli-Q Biocel A10 purification unit. Cuprisorb was purchased from Seachem. Ethanol (96.0%, EtOH), mEthanol (98.5%, MeOH) and acetonitrile (99.9%, ACN) were obtained from VWR international. NaCl (99%) was purchased from Alfa Aesar (FR). Azide monosaccharides (β-Dgalactopyranosyl azide, Gal-N 3 ; and β-D-glucopyranosyl azide (Glu-N 3 ) were obtained from Carbosynth (UK). Ammonium Acetate and Ammonium pyrrolidinedithiocarbamate, APDC, were purchased from Fisher Scientific (FR). RCA 120 and RCA 120 -Fluorescein were purchased from Eurobio (FR). Human serum from human male AB plasma, USA origin, sterile-filtered, was used as received from Sigma-Aldrich (FR). N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMDETA) was purchased from Sigma-Aldrich (FR). CuSO 4 •5H 2 O was obtained from VWR (FR). Sodium ascorbate was obtained from Fisher Scientific (FR).

Anaya L.M.B., Petitdemange R., Rosselin M., Ibarboure E., Garbay B., Garanger E., Deming T.J, & Lecommandoux S. (2021). Design of Thermoresponsive Elastin-Like Glycopolypeptides for Selective Lectin Binding and Sorting. Biomacromolecules, 22(1).

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Recombinant Histone Protein Expression

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Human HIRIP3, H2A, H2A.Z and H2B cDNAs were PCR-amplified and sub-cloned either in a home-made bi-cistronic pET 28b or pGEX-5X1 vectors, which are under the control of T7 promoter [22 (link)]. PCR-directed mutagenesis was used to generate all the described mutations or deletions. Constructs were introduced into competent BL21-CodonPlus-RIL bacteria (Stratagene, San Diego, CA, USA) using standard heat-shock transformation procedure [23 (link)]. Transformed cells were grown in Luria–Bertani broth at 37 °C, with appropriate antibiotics, till an absorbance of 0.5 at 600 nm. Expression was induced by adding a final concentration of 1 mM isopropyl-β-d-thiogalactopyranoside (Euromedex, Souffelweyersheim, France), which activates expression of genes under control of T7 promoter. After induction, cells were further grown for 2 h at 25 °C. Cell pellets were collected by low-speed centrifugation (10 min at 5000 rpm) and stored at 80 °C.

Ignatyeva M., Patel A.K., Ibrahim A., Albiheyri R.S., Zari A.T., Bahieldin A., Bronner C., Sabir J.S, & Hamiche A. (2024). Identification and Characterization of HIRIP3 as a Histone H2A Chaperone. Cells, 13(3), 273.

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Recombinant Protein Expression in E. coli

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LB medium was purchased from Sigma-Aldrich (FR). Bacto-tryptone, and yeast extract were purchased from Biokar Diagnostics (FR). Ampicillin was obtained from Eurobio (FR). Glycerol and isopropyl β-D-thiogalactopyranoside (IPTG) were purchased from Euromedex (FR).
Complete mini EDTA-free protease inhibitors were purchased from Roche Diagnostics (D).
Bis(trifluoromethane)sulfonamide lithium salt was purchased from TCI Europe (BEL). Sodium

Petitdemange R., Garanger E., Bataille L., Bathany K., Garbay B., Deming T.J, & Lecommandoux S. (2017). Tuning Thermoresponsive Properties of Cationic Elastin-like Polypeptides by Varying Counterions and Side-Chains. Bioconjugate chemistry, 28(5).

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Reagents for Bacterial Culture Experiments

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Materials. LB medium was purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, FR). Bacto-tryptone, and yeast extract were purchased from Biokar Diagnostics (Allone, FR). Ampicillin was obtained from Eurobio (Courtaboeuf, FR). Glycerol and isopropyl β-D-thiogalactopyranoside (IPTG) were purchased from Euromedex (Souffelweyersheim, FR). Complete mini EDTA-free protease inhibitors were purchased from Roche Diagnostics (Mannheim, D). Hydrogen peroxide and formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glacial acetic acid was obtained from Fisher Scientific (USA). Deionized water (18 MΩ•cm) was obtained by passing in-house deionized water through a Millipore Milli-Q Biocel A10 purification unit.

Petitdemange R., Garanger E., Bataille L., Dieryck W., Bathany K., Garbay B., Deming T.J, & Lecommandoux S. (2017). Selective Tuning of Elastin-like Polypeptide Properties via Methionine Oxidation. Biomacromolecules, 18(2).

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