Folded capillary cell dts1060

The particle sizes and zeta potentials were determined by a Zetasizer Nano ZS at 20 °C. The instrument contains a 4 mW He-Ne laser operating at a wave length of 633 nm and incorporated noninvasive backscatter optics (NIBS). The measurements were performed at the detection angle of 173° and the software automatically determined the measurement position within the quartz cuvette. The 10 -3 -10 -5 M aqueous solutions of 2, vitD3 (dissolved in ethanol 1 × 10 -3 , 1 × 10 -2 and 1 × 10 -1 M) and vitD3 with macrocycle 2 complex were prepared. The concentration ratio of pillar[5]arene 2 and vitD3 in the complex was 1:1, 1:5, 1:10 and 2:1. Electrophoretic mobility of different samples was using a fold capillary cuvette (Folded Capillary Cell DTS1060, Malvern, U.K.). The experiments were carried out for each solution in triplicate.

Freshly prepared NP solutions were used for physicochemical analysis (Luo et al., 2010) (link). The mean particle size and PDI of the NP formulations were determined by dynamic light scattering (DLS). The ZP values were measured with the use of laser doppler velocimetry (LDV). Both DLS and LDV analysis were performed in triplicate at 25 °C with a Zetasizer Nano series Nano-ZS ZEN3600 fitted with a 633 nm laser (Malvern Instruments Ltd., UK), using a folded capillary cuvette (Folded capillary cell-DTS1060, Malvern, UK). The values presented herein were acquired from three separate experiments, each of which included three replicates; N=3.