Female Lewis rats (age, 8 weeks; body weight, 200–250 g) purchased from the Charles River Laboratories Japan (Yokohama, Japan) were anesthetized with a mixture of Dormicam (4 mg/kg), Vetorphale (5 mg/kg), and Domitor (4 mg/kg), and laminectomy was performed at the thoracic 10 (Th10) vertebral level, where the spinal cord was exposed. A microsyringe (75RN, Hamilton, Reno, NV, USA) was inserted 1.5 mm at Th10 and used to infuse patient-derived AQP4 recombinant antibody [12 (link)] or control IgG [Human IgG1 lambda, Nordic-MUbio BV, Susteren, Netherlands] (20 μg), 1 μL of normal human serum, and indocyanine green (Tokyo Chemical Industry, Tokyo, Japan). Total volume was 4 μL. Rats in the TNP-ATP–treated group received a mixture of 1 μg TNP-ATP (SM0740, Sigma-Aldrich, St. Louis, MO, USA). All experimental procedures were performed according to the protocols approved by the Animal Care and Use Committee of Osaka University School of Medicine and the US Public Health Service Policy on Humane Care and Use of Laboratory Animals.
Dormicam
Manufactured by Charles River Laboratories
Sourced in Japan
Dormicam is a laboratory equipment product designed for monitoring and recording animal movement and behavior. It utilizes motion detection technology to capture detailed data on the activity patterns of small laboratory animals, such as rodents, during their resting or sleep cycles.
Automatically generated - may contain errors
2 protocols using dormicam
1
Spinal Cord Injury Model in Rats
2
Rat Model of Spinal Cord Injury
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Female Lewis rats (age: 8 weeks; body weight: 200-250 g) purchased from the Charles River Laboratories Japan (Yokohama, Japan) were anesthetized with a mixture of Dormicam (4 mg/kg), Vetorphale (5 mg/kg), and Domitor (4 mg/kg), and laminectomy was performed at the thoracic 10 (Th10) vertebral level, where the spinal cord was exposed. A microsyringe (Hamilton, 75RN NEURO SYRINGE, Cat.
No.4015-63014) was inserted 1.5 mm at Th10 and used to infuse patient-derived AQP4 recombinant antibody [10] or control IgG [Nordic-MUbio, Human IgG1 lambda G1m(f)] (20μg), 1 µL of normal human serum, and indocyanine green (Tokyo Chemical Industry, I0535). Total volume was 4 μL. Rats in the TNP-ATP-or anti-IL-1β antibody-treated group received a mixture of 1 μg TNP-ATP (Sigma-Aldrich, SM0740) or 1 μL anti-IL-1β antibody (BioXCell, BE0246). Rats in the minocycline-treated group received three doses of 80 mg/kg minocycline (Sigma-Aldrich, USA, M9511) on postoperative days 0, 1, and 2. All experimental procedures were performed according to the protocols approved by the Animal Care and Use Committee of Osaka University School of Medicine and the US Public Health Service Policy on Humane Care and Use of Laboratory Animals.
No.4015-63014) was inserted 1.5 mm at Th10 and used to infuse patient-derived AQP4 recombinant antibody [10] or control IgG [Nordic-MUbio, Human IgG1 lambda G1m(f)] (20μg), 1 µL of normal human serum, and indocyanine green (Tokyo Chemical Industry, I0535). Total volume was 4 μL. Rats in the TNP-ATP-or anti-IL-1β antibody-treated group received a mixture of 1 μg TNP-ATP (Sigma-Aldrich, SM0740) or 1 μL anti-IL-1β antibody (BioXCell, BE0246). Rats in the minocycline-treated group received three doses of 80 mg/kg minocycline (Sigma-Aldrich, USA, M9511) on postoperative days 0, 1, and 2. All experimental procedures were performed according to the protocols approved by the Animal Care and Use Committee of Osaka University School of Medicine and the US Public Health Service Policy on Humane Care and Use of Laboratory Animals.
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