INS-1 cells (a rat insulinoma cell line) were obtained from the Chinese Academy of Sciences Shanghai Institute for Biological Sciences Cell Resource Center and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (R8758, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10 mM HEPES (H8090, Solarbio, Beijing, China), 1 mM sodium pyruvate, 50 μM β-mercaptoethanol and 10% foetal bovine serum (FBS, 10099-141, Gibco, Thermo Fisher, Shanghai, China) at 37℃ with 5% CO2. For cells with hypericin treatment, we prevented photoactivation of hypericin by covering the cell culture dish with tin foil and switching off the lights when manipulating cells in the culture hood.
H8090
Manufactured by Solarbio
Sourced in China
The H8090 is a laboratory equipment designed for basic research and analysis tasks. It serves as a versatile tool for various scientific applications. The core function of the H8090 is to provide reliable and consistent performance in the laboratory environment.
Automatically generated - may contain errors
2 protocols using h8090
1
Culturing INS-1 Cells with Hypericin
2
Osteogenic Differentiation of hBMSCs from Femoral Head Necrosis Patients
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The hBMSCs from patients with femoral head necrosis secondary to senile femoral neck fractures were selected as the experimental subjects. Cells were seeded on 6-well plates. When cells reached 60–70% confluence, the medium was changed with the osteogenic differentiation medium to induce differentiation. The osteogenic differentiation medium was freshly prepared before use, which contained basic medium, 10% fetal bovine serum, 10 nmol/L dexamethasone (D8040; Beijing Solarbio Co., Ltd., China), 10 nmol/L ascorbic acid (A8100; Beijing Solarbio Co., Ltd., China), 50 mol/mL of glycerol phosphatide (Sigma-Aldrich, USA), 1% penicillin–streptomycin and 1% HEPES (H8090; Beijing Solarbio Co., Ltd., China). After 21 days of osteogenic differentiation, hBMSCs in the 6-well plate were washed with PBS and fixed with neutral formalin for 30 min. The fixation fluid was discarded, and the cells were rewashed with PBS. One milliliter of ARS agent (G8550; Beijing Solarbio Co., Ltd., China) was added to the wells for staining for 5 min. Then cells were washed and dried. The staining effect was observed under a microscope (DYF-880; Shanghai Dianying Optical Instrument Co., Ltd., China).
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