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Anti mouse inos mab

Manufactured by Cell Signaling Technology
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Sourced in United States

Anti-mouse iNOS mAb is a monoclonal antibody that recognizes inducible nitric oxide synthase (iNOS) in mouse samples.

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Lab products found in correlation

Vectashield medium with dapi, by Vector Laboratories (2 mentions) Anti mouse cd206 mab, by Cell Signaling Technology (2 mentions) Goat anti mouse texas red conjugated igg, by Merck Group (2 mentions) Rabbit anti mouse lc3b mab, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Anti-iNOS (69 citations)

2 protocols using anti mouse inos mab

1

Immunofluorescence Staining of KCs

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LC3B, iNOS, and CD206 in KCs were identified by indirect immunofluorescence staining using rabbit anti-mouse LC3B mAb, anti-mouse iNOS mAb, and anti-mouse CD206 mAb (Cell Signaling Technology, MA, USA). After washing, the KCs which were premounted with VECTASHIELD medium with DAPI (Vector) were incubated with secondary antibody goat anti-mouse Texas Red-conjugated IgG (Sigma, Saint Louis, MO, USA). The numbers of positive-stained cells were counted blindly in 10 high-power field (HPF) sections.
Ding W., Duan Y., Qu Z., Feng J., Zhang R., Li X., Sun D., Zhang X, & Lu Y. (2021). Acidic Microenvironment Aggravates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating M1-Polarization Through Regulating PPAR-γ Signal. Frontiers in Immunology, 12, 697362.
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2

Immunofluorescent Quantification of KC Markers

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LC3B, iNOS, and CD206 in KCs were identified by immunofluorescence using rabbit anti-mouse LC3B mAb, anti-mouse iNOS mAb, and anti-mouse CD206 mAb (Cell Signaling Technology, MA, USA). After incubation with secondary goat anti-mouse Texas Red-conjugated IgG (Sigma, Saint Louis, MO, USA), the KCs were pre-mounted with VECTASHIELD medium with DAPI (Vector). Positive cells were counted blindly in 10 HPF/section (200×). The positive cells were expressed as a percentage of total cells.
Zhou S., Gu J., Liu R., Wei S., Wang Q., Shen H., Dai Y., Zhou H., Zhang F, & Lu L. (2018). Spermine Alleviates Acute Liver Injury by Inhibiting Liver-Resident Macrophage Pro-Inflammatory Response Through ATG5-Dependent Autophagy. Frontiers in Immunology, 9, 948.
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