Pancreatic samples were first fixed in freshly prepared 4% paraformaldehyde and embedded in paraffin. Sections were dewaxed, rehydrated, and blocked in 10% normal donkey serum for 2 h. Sections were then incubated with an anti-Isl1 antibody diluted 1:150 (AF1837; R&D, USA) at 4 °C overnight before being rinsed three times and incubated with a donkey anti-goat antibody diluted 1:200 (DAR-488, Life Technologies, USA) for 3 hours at room temperature. After three rinses and overnight incubation with an anti-PIASy antibody diluted 1:200 (sc-166706, Santa Cruz Biotechnology, USA) at 4 °C, the sections were rinsed three times and incubated with a donkey anti-mouse antibody diluted 1:200 (DAM-555, Life Technologies, USA) for 3 hours at room temperature. After three final rinses, the sections were examined under a fluorescence microscope (Leica Microsystems, Cambridge, UK). The NIT cell immunofluorescence assay was performed as previously described7 (link).
Sc 166706
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-166706 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and experimentation. The core function of this product is to [DESCRIPTION_NOT_AVAILABLE].
Automatically generated - may contain errors
2 protocols using sc 166706
1
Immunofluorescence Staining of Pancreatic Tissue
2
Western Blotting Protocol for Cardiac Tissue
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After 2 h of reperfusion, rat ventricular tissues in the peri-infarct region were harvested, and the procedure for Western blotting was performed as described previously.[14 (link)] The primary antibodies used in this study were as follows: anti-Cx43 (1:1000, #3512S, Cell Signaling Technology), anti-phospho-Cx43-S368 (1:1000, #3511S, Cell Signaling Technology), anti-SUMO2/3 (1:500, ab3754, abcam, USA), anti-PIASy (1:1000, sc-166706, Santa Cruz Technology, Beverly, MA, USA), anti-voltage-gated Sodium Channel 1.5 (NaV1.5) (1:200, GTX54798, Gentex, San Antonio, TX, USA), and anti-plakophilin 2 (PKP2) (1:1000, GTX54579, Gentex). The secondary antibody (1:5000) was purchased from Cell Signaling Technology.
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