An amount of 0.5 ml of water was removed from each tube and mixed with the same volume of TISAB II (total ionic strength adjustment buffer solution) to occur F dissociation. The F concentration was determined with a specific ion electrode (Orion 9606, Thermo Fisher Scientific, Waltham, USA) coupled to a digital potentiometer (EA-940, Thermo Fisher Scientific, Waltham, USA) using standards with 0.05 to 8 μg F/mL for the standard calibration. The results were expressed in μg F/mL. Additionally, the area under the curve (AUC) of the F release (μg F/mL) as a function of time (days) was calculated using the program Origin 217 (One Round house Plaza, Northampton, MA, USA).
Ea 940
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EA-940 is an elemental analyzer manufactured by Thermo Fisher Scientific. It is designed to determine the elemental composition of organic and inorganic materials through the analysis of carbon, hydrogen, nitrogen, and sulfur content.
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Lab products found in correlation
2 protocols using ea 940
1
Fluoride Determination Protocol
2
Fluoride Influence on Erosion Prevention
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The concentration of fluoride in each test substance was evaluated in order to assess any possible of the influence of fluoride in preventing erosion.
The solutions prepared in the laboratory were added to TISAB II (1.0 M acetate buffer, pH 5.0, containing 1.0 M NaCl, and CDTA at 0.4%) in a 1:1 proportion. The analyses were performed in duplicate. Fluoride (F -) determination was performed with a specific electrode (ORION 96-06; Thermo Scientific, Inc.) and an ion analyzer (EA 940, Thermo Scientific) that was previously calibrated with standards containing 0.1 to 2 µg of F -/mL prepared from a 100 ppm F -standard (ORION 940907, Thermo Scientific).
The solutions prepared in the laboratory were added to TISAB II (1.0 M acetate buffer, pH 5.0, containing 1.0 M NaCl, and CDTA at 0.4%) in a 1:1 proportion. The analyses were performed in duplicate. Fluoride (F -) determination was performed with a specific electrode (ORION 96-06; Thermo Scientific, Inc.) and an ion analyzer (EA 940, Thermo Scientific) that was previously calibrated with standards containing 0.1 to 2 µg of F -/mL prepared from a 100 ppm F -standard (ORION 940907, Thermo Scientific).
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