Open-circuit indirect calorimetry was performed with an O2/CO2 metabolism measuring system for small animals (MK-5000RQ; Muromachi Kikai, Tokyo, Japan). The system monitored oxygen consumption (VO2) and carbon dioxide production (VCO2) at 3-min intervals and calculated the respiratory quotient (RQ) ratio (VCO2/VO2). Spontaneous motor activity was measured using the Supermex infrared sensor (Muromachi Kikai). Measurements were performed for the dark (from 19:00 to 7:00) or light (from 7:00 to 16:30) period under ad libitum feeding conditions. The substrate utilization rate and energy production rate were calculated using the formula used by Ferrannini [35 (link)] where the rate of glucose oxidation (g/min) = 4.55VCO2 (L/min)-3.21VO2(L/min)-2.87 N(mg/min), the rate of lipid oxidation (g/min) = 1.67 (VO2-VCO2)-1.92N, and the rate of energy production (kcal/min) = 3.91VO2 + 1.10VCO2-3.34N, where N is the rate of urinary nitrogen excretion used to estimate protein oxidation. However, considering that only a small portion of resting and exercise energy expenditure arises from protein oxidation [36 (link)], the contributions of protein oxidation were neglected.
Supermex infrared sensor
Manufactured by Muromachi Kikai
The Supermex infrared sensor is a device designed to detect and measure infrared radiation. It is a core component used in various applications that require infrared sensing capabilities. The sensor's function is to convert infrared radiation into electrical signals, which can then be processed and analyzed.
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Lab products found in correlation
2 protocols using supermex infrared sensor
1
Indirect Calorimetry of Metabolic Parameters
2
Indirect Calorimetry in Mice
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Open-circuit indirect calorimetry was performed with an O 2 /CO 2 metabolism measuring system for small animals (MK-5000RQ; Muromachi Kikai Co., Ltd) 1 week before execution as described previously (29) . The system monitored VO 2 and carbon dioxide production (VCO 2 ) at 3-min intervals and calculated the RQ ratio (VCO 2 :VO 2 ). Spontaneous motor activity was measured using a Supermex infrared sensor (Muromachi Kikai Co., Ltd). Measurements were performed for the dark (from 19.00 to 07.00 hours) and light (from 07.00 to 16.30 hours) periods under ad libitum feeding conditions. The energy production rate was calculated with the formulae used by Ferrannini in which the rate of energy production , where N is the rate of urinary nitrogen excretion used to estimate protein oxidation (32) . However, considering that only a small portion of the resting and exercise energy expenditure arises from protein oxidation, the contributions of protein oxidation were neglected. These measurements were only done on the HF mice.
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