DNA was extracted from adult female L. fabarum in 10 sub-samples (50–100 wasps each) using the QIAmp DNA mini Kit (Qiagen) according to the manufacturer’s instructions, with the inclusion of an overnight tissue digestion at 56 °C. Extracted DNA was then pooled and used to produce Illumina PE and MP, and PacBio libraries. The PE library was prepared using the Illumina Paired-End DNA protocol; the average fragment size was 180 base pairs (bp). The MP library (5 kb insert) was generated with the Nextera mate-pair protocol (Illumina). Both libraries were sequenced on the Illumina MiSeq in Paired-End mode at the Functional Genomics Center Zürich.
Long-read libraries for PacBio RS II sequencing were produced using the DNA Template Prep Kit 2.0 (Pacific Biosciences). Input DNA was mechanically sheared to an average size distribution of 10Kb (Covaris gTube, Kbiosciences) and the resulting library was size selected on a Blue Pippin Size Selection System (Sage Science) machine to enrich fragments >8Kb; quality and quantity were checked on the Bioanalyzer and Qubit, respectively. Ten SMRT Cells were sequenced at the Functional Genomics Center Zürich.
Long-read libraries for PacBio RS II sequencing were produced using the DNA Template Prep Kit 2.0 (Pacific Biosciences). Input DNA was mechanically sheared to an average size distribution of 10Kb (Covaris gTube, Kbiosciences) and the resulting library was size selected on a Blue Pippin Size Selection System (Sage Science) machine to enrich fragments >8Kb; quality and quantity were checked on the Bioanalyzer and Qubit, respectively. Ten SMRT Cells were sequenced at the Functional Genomics Center Zürich.