Sp600125

Manufactured by AG Scientific

Sourced in United States

SP600125 is a potent and selective inhibitor of the c-Jun N-terminal kinase (JNK) signaling pathway. It functions by blocking the catalytic activity of the JNK enzyme, which plays a crucial role in various cellular processes, including cell growth, differentiation, and apoptosis. This product is commonly used in research applications to investigate the biological functions and signaling mechanisms mediated by the JNK pathway.

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Lab products found in correlation

Sb203580, by AG Scientific (3 mentions) Cobalt protoporphyrin copp, by Enzo Life Sciences (2 mentions) Ly294002, by AG Scientific (2 mentions) Pd98059, by AG Scientific (2 mentions) Proteinase k, by Thermo Fisher Scientific (2 mentions) Rabbit polyclonal antibody against β actin, by Abcam (1 mentions) Rabbit polyclonal antibodies against iκb α, by Santa Cruz Biotechnology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Sb202190, by AG Scientific (1 mentions) Recombinant human il 1β, by R&D Systems (1 mentions) Pepinh myd, by InvivoGen (1 mentions) Cli 095, by InvivoGen (1 mentions) Oxpapc, by InvivoGen (1 mentions) Pd98059, by Selleck Chemicals (1 mentions) Sb203580, by Selleck Chemicals (1 mentions) Crocin, by Merck Group (1 mentions) Kn 92, by Cayman Chemical (1 mentions) Kn 62, by Cayman Chemical (1 mentions) Actinomycin d actd, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

5 protocols using sp600125

Osteoclastogenesis Regulation Pathway

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Recombinant human M-CSF and mouse RANKL proteins were prepared as previously described⁵ (link). BA was purchased from AK Scientific, Inc. (Union City, CA, USA) and recombinant human IL-1β was supplied by R&D systems, Inc. (Minneapolis, MN, USA). PGE2 was purchased from Cayman Chemical (Ann Arbor, MI, USA). Lipopolysaccharide (LPS), U0126 and SC-514 were supplied by MilliporeSigma (Burlington, MA, USA). SB202190 and SP600125 were supplied by AG Scientific, Inc. (San Diego, CA, USA). A rabbit polyclonal antibody against β-actin was purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal antibodies against phosphorylated (p-)IκBα, p-JNK, p-ERK and p-P38, and a rabbit monoclonal antibody against p-p65 were purchased from Cell Signaling Technology (Danvers, MA, USA). A goat polyclonal anti-mouse secondary antibody (Alexa Fluor 546 conjugate) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Rabbit polyclonal antibodies against IκBα, JNK1, P38 and c-FOS, and mouse monoclonal antibodies against NFATc1, p65, ERK2, CTSK and integrin β₃ were procured from Santa Cruz Biotechnology (Dallas, TX, USA). A rabbit polyclonal antibody against superoxide dismutase 2 (SOD2) was purchased from Upstate Biotechnology (Lake Placid, NY, USA). A rabbit monoclonal antibody against ATP6V0D2 was kindly provided by Dr. SY Lee (Ewha Womans Univ. Seoul, Korea).

Son H.S., Lee J., Lee H.I., Kim N., Jo Y.J., Lee G.R., Hong S.E., Kwon M., Kim N.Y., Kim H.J., Park J.H., Lee S.Y, & Jeong W. (2019). Benzydamine inhibits osteoclast differentiation and bone resorption via down-regulation of interleukin-1β expression. Acta Pharmaceutica Sinica. B, 10(3), 462-474.

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Protease and Inhibitor Procurement

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Proteinase K was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Bay11-7082 and SP600125 were purchased from A.G. Scientific (San Diego, CA, USA). PD98059 and SB203580 were purchased from Selleck (Houston, TX, USA). Pepinh-MYD, OxPAPC, and CLI-095 were purchased from Invivogen (San Diego, CA, USA).

Jeon J., Lee Y., Yu H, & Ha U. (2021). Pseudomonas aeruginosa DnaK Stimulates the Production of Pentraxin 3 via TLR4-Dependent NF-κB and ERK Signaling Pathways. International Journal of Molecular Sciences, 22(9), 4652.

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Protoberberine Derivatives Screening

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Each of 80 protoberberine derivatives was dissolved in DMSO to a final concentration of 1 mg/ml. A stock solution containing 1 mg/ml of SP600125, SB203580, or U0126 (A.G. Scientific Inc.) was also prepared in DMSO.

Kim N., Park J., Gadhe C.G., Cho S.J., Oh Y., Kim D, & Song K. (2014). A Protoberberine Derivative HWY336 Selectively Inhibits MKK4 and MKK7 in Mammalian Cells: The Importance of Activation Loop on Selectivity. PLoS ONE, 9(4), e91037.

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Investigating Crocin and LPS-Induced Cellular Responses

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Crocin (Figure 1(a)), LPS (phenol extracted from Salmonella enteritidis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), actinomycin D (Act. D), cycloheximide (CHX), 2-(3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid acetoxymethyl ester (Fluo-3/AM), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP) were bought from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). LY294002, PD98059, SP600125, and SB203580 were obtained from A.G. Scientific (San Diego, CA, USA). Nifedipine, 1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM), and calmidazolium chloride were acquired from Calbiochem (EMD Millipore, Billerica, MA, USA). KN92, KN93, and KN62 were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Calbiochem.

Kim J.H., Park G.Y., Bang S.Y., Park S.Y., Bae S.K, & Kim Y. (2014). Crocin Suppresses LPS-Stimulated Expression of Inducible Nitric Oxide Synthase by Upregulation of Heme Oxygenase-1 via Calcium/Calmodulin-Dependent Protein Kinase 4. Mediators of Inflammation, 2014, 728709.

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Anti-inflammatory Effects of Dioscorea moniliforme Extract

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Cobalt protoporphyrin (CoPP) was purchased from Enzo Life Sciences, Inc. (USA). Actinomycin D (AD), cycloheximide (CHX), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and LTA from Staphylococcus aureus were purchased from Sigma-Aldrich Co. (USA). LY294002, PD98059, SP600125 and SB203580 were obtained from A.G. Scientific (USA). The antibodies for iNOS, HO-1, Nrf2, Akt, p38, TATA-box binding protein (TBP) and histone deacetylases (HDAC) were purchased from Santa Cruz Biotechnology (USA), the antibodies for phospho-Akt and phospho-p-38 were purchased from Cell Signaling Technology (USA), and the anti-tubulin antibody was purchased from Bio Genex (USA). The dry shoot systems of D. moniliforme Sw. were obtained and extracted as described previously [30] . In brief, the dry shoot systems (300 g) were extracted with distilled water at 100ºC for 4 h. The extract was filtered through a 0.45-μm filter, freeze-dried (yield: 8 g), and stored at 4ºC. The dried extract was dissolved in phosphate-buffered saline (PBS) and filtered through a 0.22 μm-filter before use.

Lee Y.J., Kim J.H, & Kim Y. (2018). Dendrobium moniliforme Stem Extract Inhibits Lipoteichoic Acid-Induced Inflammatory Responses by Upregulation of Heme Oxygenase-1. Journal of microbiology and biotechnology, 28(8).

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