Tris(hydroxymethyl)-aminomethane (tris), boric acid, acetic acid, KCl, EDTA, acrylamide/bisacrylamide (19:1), ammonium persulfate (prepared as a 10% solution in sterile water and stored at 4°C) and tetramethylethylenediamine (TEMED) were purchased from Fisher Scientific. Magnesium chloride hexahydrate, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) and 30% ammonia water were purchased from Arcos Organics. Sodium chloride, ethidium bromide, streptavidin, protocatechuic acid (PCA), MES monohydrate, sodium hydroxide and hydrogen peroxide were purchased from VWR. Bovine serum albumin (BSA) was purchased from New England Biolabs (NEB). Protocatechuate 3,4-dioxygenase (PCD) was purchased from MP Biomedicals, and suspended in a pH 8.0 PCD buffer (100 mM Tris-HCl, 50 mM KCl, 1 mM EDTA, 50% glycerol) at 0.2 μM, sterile filtered and stored at −20°C. Biotin-modified BSA was purchased from Peirce, dissolved in sterile H2O at 1 mg/ml, sterile filtered and stored at −20°C. Catalase from bovine liver and glucose oxidase from Asperigillus niger were purchased from Sigma Aldrich. For nanopore experiments, hexadecane, pentane, potassium chloride and Tris were purchased from Sigma-Aldrich. 1,2 diphytanoyl-sn-glycero-3-phosphocholine (DPhyPC) lipid was purchased from Avanti polar lipids. Alpha toxin from Staphylococcus Aureus was purchased from List Biological.
1 2 diphytanoyl sn glycero 3 phosphocholine dphypc lipid
Manufactured by Avanti Polar Lipids
Sourced in United States
1,2 diphytanoyl-sn-glycero-3-phosphocholine (DPhyPC) is a synthetic lipid compound commonly used in liposome and membrane research applications. It is a phospholipid with a choline headgroup and two branched phytanoyl fatty acid chains. DPhyPC is known for its ability to form stable lipid bilayers and is often used as a model system to study membrane properties and behaviors.
Automatically generated - may contain errors
Lab products found in correlation
Potassium chloride (kcl), by Merck Group (2 mentions)
Hexadecane, by Merck Group (2 mentions)
Sodium chloride (nacl), by Avantor (1 mentions)
Ammonium persulfate, by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide, by Avantor (1 mentions)
Tetramethylethylenediamine, by Thermo Fisher Scientific (1 mentions)
Ethidium bromide, by Avantor (1 mentions)
Catalase from bovine liver, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Protocatechuic acid pca, by Avantor (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Glucose oxidase from asperigillus niger, by Merck Group (1 mentions)
Protocatechuate 3 4 dioxygenase pcd, by MP Biomedicals (1 mentions)
Bovine serum albumin (bsa), by New England Biolabs (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Acrylamide bis acrylamide 19 1, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Pentane, by Merck Group (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
2 protocols using 1 2 diphytanoyl sn glycero 3 phosphocholine dphypc lipid
1
Nanopore Sensing Reagent Preparation
2
Formation of Lipid Bilayer Membranes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1,2-Diphytanoyl-sn-glycero-3-phosphocholine
(DPhyPC) lipid was purchased from Avanti Polar Lipids (Alabaster,
AL, USA). Alpha toxin from Staphylococcus aureus was purchased from IBT Bioservices (Rockville, MD, USA). Teflon
supports for membrane formation were purchased from Goodfellow USA
Corp. (Coraopolis, PA, USA). Holes (50 μm diameter) were formed
in the Teflon sheets with laser drilling at Potomac Photonics Inc.
(Halethorpe, MD, USA). Borosilicate glass capillaries (with filament)
were purchased from Sutter Instruments (Novato, CA, USA). Potassium
tetrachloroaurate (III) hydrate, Tris, tiopronin, potassium chloride,
hexadecane, citric acid, and potassium hydroxide were purchased from
Sigma-Aldrich (St. Louis, MO, USA). The borate-tert-butylamine complex was purchased from Alfa Aesar (Ward Hill, MA,
USA). n-Pentane and methanol were purchased from
Fisher Scientific (Washington, DC, USA). All peptides were purchased
from GenScript (Piscataway, NJ, USA). All chemicals were used as received
without further purification. Urine was collected from a female donor
using informed consent, in accordance with the approved Institutional
Review Board Human Subjects Research Protocol (HM20002931). Urine
was deposited into a sterile collection cup supplied to the donor
and returned on ice within 24 h before aliquoting into 1 mL volumes
and freezing at −80 C until use.
(DPhyPC) lipid was purchased from Avanti Polar Lipids (Alabaster,
AL, USA). Alpha toxin from Staphylococcus aureus was purchased from IBT Bioservices (Rockville, MD, USA). Teflon
supports for membrane formation were purchased from Goodfellow USA
Corp. (Coraopolis, PA, USA). Holes (50 μm diameter) were formed
in the Teflon sheets with laser drilling at Potomac Photonics Inc.
(Halethorpe, MD, USA). Borosilicate glass capillaries (with filament)
were purchased from Sutter Instruments (Novato, CA, USA). Potassium
tetrachloroaurate (III) hydrate, Tris, tiopronin, potassium chloride,
hexadecane, citric acid, and potassium hydroxide were purchased from
Sigma-Aldrich (St. Louis, MO, USA). The borate-tert-butylamine complex was purchased from Alfa Aesar (Ward Hill, MA,
USA). n-Pentane and methanol were purchased from
Fisher Scientific (Washington, DC, USA). All peptides were purchased
from GenScript (Piscataway, NJ, USA). All chemicals were used as received
without further purification. Urine was collected from a female donor
using informed consent, in accordance with the approved Institutional
Review Board Human Subjects Research Protocol (HM20002931). Urine
was deposited into a sterile collection cup supplied to the donor
and returned on ice within 24 h before aliquoting into 1 mL volumes
and freezing at −80 C until use.
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