Specific elisa

For measurement of cytokine secretion, human T cells (10⁶ cells/mL) were seeded in anti-CD3/CD28 antibody-coated 24-well plates (Corning Inc., Amsterdam, The Netherlands) and treated with increasing concentrations of Pitavastatin or vehicle control. Supernatants were harvested after 72 h, and cytokine concentrations (IFN-γ, IL-5, IL-10 and IL-17) were determined with specific ELISA (bio-techne Ltd., Minneapolis, MN, USA), according to manufacturer’s instructions. The optical density of each well was determined at 450 and 570 nm (Tecan Safire Microplate Reader, Tecan Group, Männedorf, Switzerland). The concentration of each cytokine was calculated by using a standard curve and the measured absorbance (n = 4 independent experiments).

For measurement of cytokine secretion, human T cells (10⁶ cells/ml) were seeded in anti CD3/CD28 antibody coated 24-well plates (Corning Inc.) and treated with increasing concentrations of ADV or vehicle control. Supernatants were harvested after 72 h and cytokine concentrations (IFN-γ, IL-5, IL-10, and IL-17) were determined with specific ELISA (Bio-techne Ltd., Minneapolis, MN, USA) according to manufacturer’s instructions. The optical density of each well was determined at 450 and 570 nm (Tecan Safire Microplate Reader, Tecan Group, Männedorf, Switzerland). The concentration of each cytokine was calculated using a standard curve and the measured absorbance.