Piperacillin

Peptides were purchased from GL Biochemistry Inc. (Shanghai, China). The purity of peptides was ≥5%. Amino acid sequences and physical characteristics for peptides are shown in Supplementary Table S1. TRIzol reagent and Super Script^TM III kits were purchased from TaKaRa Inc. (Dalian, China). Ceftazidime, piperacillin and levofloxacin were purchased from Solarbio Inc. (Beijing, China). C4-HSL standards is purchased from Sigma (Sigma, China). All other reagents were of analytical grade. The MDR P. aeruginosa (MRPA) strain 0108 (MRPA0108) was obtained from the Clinical Laboratory Department, the First Affiliated Hospital of Dalian Medical University. MRPA0108 exhibits resistance to gentamicin, kanamycin, ampicillin, cefepime, streptomycin, tetracycline, amoxicillin, and ciprofloxacin. Identification and antimicrobial susceptibility testing (AST) of MRPA0108 were performed by an automated MicroScan WalkAway 96 Plus system (Siemens Ltd., Germany) at the First Affiliated Hospital of Dalian Medical University.

Penicillin G, Piperacillin, Ceftriaxone, Cefepime, Cefazolin, Meropenem were purchased from Solarbio Co., Ltd. (Beijing, China). Imipenem was bought from Fresenius Kabi (Bad Homburg, Germany). HEPES was obtained from Sangon Biotech Co., Ltd. (Shanghai, China). All other reagents were purchased from Sigma-Aldrich Co (St. Louis, USA). The controlled pore glass (CPG) beads (125–140 μm particle diameter and 50 nm pore size) were originally purchased from VEB Trisola, Steinach, Germany. The construction of plasmid encoding NDM-1, expression and purification of recombinant NDM-1(rNDM-1) were performed as previously described (Meng et al., 2021a (link); Meng et al., 2021b (link)). The purity of the purified rNDM-1 is > 95%, The unit activity of rNDM-1 to hydrolyze Meropenem is 108 IU/mg.