Every mouse in each group was sacrificed to obtain the spleen according to the method in Gottrand et al.'s study (41 (link)) at postoperative days 3, 7, and 14, respectively. Before the sacrificion, nylon fiber column (Polysciences, PA, USA) was incubated for 1 h using 1,640 culture medium containing 10% fetal bovine serum (FBS) (Gibco, MA, USA). The mice were sacrificed by carbon dioxide inhalation and then immersed in 75% alcohol for 10 min to be sterilized. Their spleens were obtained and grinded on 200-mesh stencil aseptically. Grinding fluid was centrifuged in 900 rpm for 5 min, the supernatant was abandoned, and 2 ml 1X red blood cell lysis buffer was added. The solution was then centrifuged again, the supernatant was abandoned, the deposit was resuspended by PBS, and then, cells were collected into the culture flask after transiting nylon fiber columns.
Nylon fiber column
Manufactured by Polysciences
Sourced in United States
The Nylon fiber column is a laboratory equipment used for various applications. It is designed to facilitate the separation, purification, and analysis of different substances. The column features a nylon-based matrix that provides efficient retention and separation capabilities. The exact specifications and intended use may vary depending on the specific application and requirements.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nylon fiber column
1
Murine Splenic Cell Isolation
2
Spleen Cell Isolation Protocol
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Every eight mice in each group were sacrificed to obtain the spleen according to the method in Gottrand et al.'s study[13 (link)] at postoperative days 3, 7, and 14, respectively. Before the sacrificion, nylon fiber column (Polysciences, PA, USA) was incubated for 1 h using 1640 culture medium containing 10% fetal bovine serum (FBS) (Gibco, MA, USA). The mice were sacrificed by carbon dioxide inhalation and then immersed in 75% alcohol for 10 min to be sterilized. Their spleen was obtained and grinded on 200-mesh stencil aseptically. Grinding fluid was centrifuged in 900 rpm for 5 min, the supernatant was abandoned, and 2 ml 1X red blood cell lysis buffer was added. The solution was then centrifuged again, the supernatant was abandoned, the deposit was resuspended by PBS, and then, cells were collected into the culture flask after transiting nylon fiber columns.
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