Heparin

Manufactured by Greiner

Sourced in Australia, Austria

Heparin is an anticoagulant medication primarily used to prevent and treat blood clots. It works by inhibiting the activation of certain blood clotting factors, thereby reducing the formation of thrombi. Heparin is commonly used in various medical procedures and treatments where the prevention of blood clots is crucial.

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Lab products found in correlation

Ficoll hypaque, by Thermo Fisher Scientific (1 mentions) Rpmi 10 complete medium, by Thermo Fisher Scientific (1 mentions) Human monocyte derived dendritic cell differentiation kit, by R&D Systems (1 mentions) Vacuatte, by Greiner (1 mentions) Vacuette tube, by Greiner (1 mentions)

Close spelling variants of this product

Vacuette® lithium-heparin tubes Lithium-heparin-containing tubes Heparin anticoagulant Vacuette Heparin Tubes Sodium-heparin tubes MiniCollect® Lithium Heparin tubes Vacuette® sodium-heparin tubes à 9 mL Lithium heparin-coated tubes Li–heparin Lithium heparin

5 protocols using heparin

Isolation and Differentiation of Human Dendritic Cells

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Blood samples were collected from Ahmed Maher teaching hospital blood bank, Egypt, in a vacutainer containing Heparin as anti-coagulant (Greiner bio-one, Australia) and processed immediately within 2 hours of collection.
The protocol was optimised for the preparation of PBMCs from human blood through using Ficoll-Hypaque density gradient centrifugation inaddition to using RPMI-10 complete medium (Gibco, United Kingdom) supplemented with penicillin/streptomycin (10 mg/ml), Glutamine (200 mM), 10% FCS, insulin and hydrocortisone.
In vitro culture and differentiation of human monocytes into dendritic cells DCs were achieved by using the Human Monocyte-derived Dendritic Cell Differentiation Kit (R&Dsystem, Minneapolis, MN, USA). This kit enables the generation of active mature DCs from human monocytes in 10 days (Amedei et al., 2011; Sabado and Bhardwaj, 2010).

Suef R. (2020). MONOCYTE-DERIVED DENDRITIC CELLS ISOLATION AND PHENOTYPIC CHARACTERIZATION. Al-Azhar Journal of Pharmaceutical Sciences/Al-Azhar Journal of Pharmaceutical Sciences, 62(2), 1-11.

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Equine Perinatal Homeostasis Study

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The study was conducted during the third trimester of pregnancy and after delivery. Foaling of mares occurred from February to the end of April. At the beginning of the study, the mares were clinically healthy and did not demonstrate any signs of systemic homeostasis disorders. The study involved blood collection and clinical observation, i.e. parturition course, leaving the placenta, and appearance of post-foaling oestrus. Blood sampling started in the perinatal period, i.e. two weeks before parturition (trial 0), within the first 24 h after delivery, and then at 7^th and 21^st day after foaling. Blood was collected from the jugular vein into sterile tubes type Vacuatte of 9 mL volume, containing heparin (Greiner Labortechnik, Austria), and delivered to the laboratory within 2 h.

Krakowski L., Bartoszek P., Krakowska I., Stachurska A., Piech T., Brodzki P, & Wrona Z. (2017). Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition. Journal of Veterinary Research, 61(2), 217-221.

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Isolation of NK Cells from Human Blood

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The study was conducted in Al-Zahraa University hospital Egypt. Human whole blood (15ml) was collected in from 10 normal, healthy, human donors ranging from 20-35 years old. blood was collected from Al-Zahraa University hospital blood bank, Egypt, in a vacutainer containing Heparin as anti-coagulant (Greiner bio-one, Australia) and processed immediately within 2 hours of collection. All donors signed a written informed agreement for the study. Differentiation of human mononuclear cells into Natural killer cells achieved by using the PBMCS isolation technique.

Suef R. (2020). EVALUATION OF NATURAL KILLER CELLS USING DIFFERENT TECHNIQUES. Al-Azhar Journal of Pharmaceutical Sciences/Al-Azhar Journal of Pharmaceutical Sciences, 62(2), 12-22.

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Intestinal Contents and Blood Sampling

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At the end of the balance trial, 2 animals per cage were randomly selected and euthanized following the principles for care of animals in experimentation. Intestinal contents were collected from the ileum (between Meckel's diverticulum and the ileocecal junction) of the 2 animals by gently finger-stripping the intestinal segment. The digesta contents collected from 2 animals per cage were pooled and stored on ice until analysis.
At the end of the performance trial, blood samples were taken from one bird per pen. The blood samples were collected in 5 mL Vacuette^® tubes containing Heparin (456,083, Greiner Bio-One GmbH, Kremsmünster, Austria), stored on ice until centrifugation (1,500 × g for 10 min) to obtain the plasma and then stored at –21°C until analysis.

Smeets N., Nuyens F., Van Campenhout L., Delezie E, & Niewold T.A. (2018). Interactions between the concentration of non-starch polysaccharides in wheat and the addition of an enzyme mixture in a broiler digestibility and performance trial. Poultry Science, 97(6), 2064-2070.

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Isolation and Preservation of PBMCs

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Blood samples were collected in Heparin and serum-gel monovette tubes (Greiner Bio-One). Peripheral blood mononuclear cells were isolated by gradient centrifugation and stored in liquid nitrogen in the presence of FCS and DMSO. Heparinized plasma and serum samples were fractioned by centrifugation, aliquoted, and stored at −80°C until analysis. Prior to experiments, aliquots of plasma samples were heat-inactivated (56°C for 30 min) and then stored at 4°C.

Wang Z., Muecksch F., Muenn F., Cho A., Zong S., Raspe R., Ramos V., Johnson B., Ben Tanfous T., DaSilva J., Bednarski E., Guzman-Cardozo C., Turroja M., Millard K.G., Tober-Lau P., Hillus D., Yao K.H., Shimeliovich I., Dizon J., Kaczynska A., Jankovic M., Gazumyan A., Oliveira T.Y., Caskey M., Bieniasz P.D., Hatziioannou T., Kurth F., Sander L.E., Nussenzweig M.C, & Gaebler C. (2022). Humoral immunity to SARS-CoV-2 elicited by combination COVID-19 vaccination regimens. The Journal of Experimental Medicine, 219(10), e20220826.

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