Anti cyclin b2

Protein preparation and Western blots were performed as described previously15 . Nuclear and cytoplasmic lysates were prepared with the NE-PER kit (Pierce Biotechnology) according to the manufacturer’s instructions. The protein levels of active β-catenin in whole-cell lysates were quantified using the anti-active β-catenin clone 8E7 antibody (Millipore). The antibody is specific for the active form of β-catenin, dephosphorylated on Ser37 or Thr41. The following primary antibodies were used: anti-menin (1:1,000; Cell Signalling Technology), anti-β-catenin (1:1,000; Cell Signalling Technology), anti-active β-catenin (1:1,000; Millipore), anti-Phospho-β-catenin (Ser33/37/Thr41; 1:1,000; Cell Signalling Technology), anti-Phospho-β-catenin (Ser45; 1:1,000; Cell Signalling Technology), anti-α-tubulin (1:1,000; Cell Signalling Technology), anti-α-tubulin (1:1,000; Cell Signalling Technology), anti-Cyclin D2 (1:1,000; Cell Signalling Technology), anti-Cyclin A (1:1,000; Abcam), anti-Cyclin B2 (1:1,000; Santa Cruz Biotechnology), anti-Mcm2 (1:1,000; Cell Signalling Technology), anti-Pbk (1:1,000; Cell Signalling Technology), anti-C-myc (1:1,000; Cell Signalling Technology), anti-GAPDH (1:10,000; KANGCHEN) and anti-lamin B (1:1,000; Santa Cruz Biotechnology). Images have been cropped for presentation. Full size images are presented in Supplementary Figs 9–11.