Anti dx5 microbeads

Manufactured by Miltenyi Biotec

Anti-DX5 microbeads are a type of magnetic separation reagent used for the isolation and enrichment of DX5-positive cells from complex biological samples. These microbeads are coated with antibodies that specifically bind to the DX5 antigen, which is expressed on the surface of certain cell types. The binding of the microbeads to the target cells allows for their magnetic separation and subsequent isolation or depletion, depending on the experimental requirements.

Automatically generated - may contain errors

Lab products found in correlation

Cytofix cytoperm kit, by BD (1 mentions)

Close spelling variants of this product

Anti-mouse DX5 microbeads PE-conjugated anti-DX5 and anti-PE microbeads

2 protocols using anti dx5 microbeads

Multiparameter Flow Cytometric Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents were from BD Biosciences, eBioscience or BioLegend: fluorochrome labeled anti-CD69 (H1.2F3), anti-CD11c (HL3), anti-CD8α (53-6.7), anti-CD4 (GK1.5), anti-I-Ab (AF6-120.1), anti-CD3 (145-2C11), anti-NK1.1 (PK136), anti-Ly49H (3D10), anti-IFN-γ (XMG1.2), anti-Granzyme B (GB11), anti-CD11b (M1/70), anti-Ly6C (AL-21), anti-Ly6G (1A8), anti-CD25 (PK61.5), anti-Foxp3 (150D/E4), anti-CD262 (DR5, MD5-1), anti-TRAIL (N2B2), and Streptavidin. The m45 tetramer used to visualize virus-specific CD8 T cells was a generous gift from M. Brown (University of Virginia, Charlottesville, VA). Fc receptors were blocked before surface staining with supernatant from HB-197 cells (ATCC). Dead cells were excluded with propidium iodide (PI). Intracellular staining for IFN-γ and Granzyme B was performed on freshly isolated spleen cells or after 4 h incubation with Brefeldin A using the BD Cytofix-Cytoperm kit (BD Biosciences) according to the manufacturer instructions. All flow cytometry was conducted on a dual laser FACSCalibur or LSR II (BD Biosciences) and analyzed with FlowJo software (Tree Star, Inc.). For NK cell purification, DX5⁺ cells were purified from spleens with anti–DX5 microbeads as recommended by the manufacturer (Miltenyi Biotec). Purities were always >85%.

Allam A., Swiecki M., Vermi W., Ashwell J.D, & Colonna M. (2014). Dual function of CD70 in viral infection: modulator of early cytokine responses and activator of adaptive responses. Journal of immunology (Baltimore, Md. : 1950), 193(2), 871-878.

+ Open protocol

+ Expand

Adoptive Transfer of NK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions of spleens from control mice were obtained as described above and NK cells were either enriched with anti-DX5 microbeads (Miltenyi Biotech) or purified by FACS based on DX5 and CD3 expression. 8×10⁶ enriched NK cells or 4×10⁶ FACS purified NK cells (>95% pure and viable) were adoptively transferred into recipient mice 1 h prior to infection.

Whitney P.G., Bär E., Osorio F., Rogers N.C., Schraml B.U., Deddouche S., LeibundGut-Landmann S, & Reis e Sousa C. (2014). Syk Signaling in Dendritic Cells Orchestrates Innate Resistance to Systemic Fungal Infection. PLoS Pathogens, 10(7), e1004276.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!