Sec498cys

Manufactured by AbFrontier

The Sec498Cys is a laboratory instrument designed for the analysis and quantification of specific biomolecules. It utilizes advanced detection methods to provide accurate and reliable results. The core function of this product is to facilitate scientific research and investigation.

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Lab products found in correlation

Aprotinin, by Merck Group (2 mentions) Leupeptin, by Merck Group (2 mentions) Bestatin, by Merck Group (2 mentions) N biotinoyl n iodoacetyl ethylenediamine biam, by Thermo Fisher Scientific (1 mentions) Enhanced chemiluminescence ecl immunoblot detection reagents, by Thermo Fisher Scientific (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphatase inhibitor cocktail, by Merck Group (1 mentions) Supersignal west pico chemiluminescense substrate kit, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions) Nadph, by Merck Group (1 mentions)

2 protocols using sec498cys

Enzymatic Assays for Oxidative Stress

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Purified rat liver TrxR1, recombinant
human Trx1, and glutathione reductase (GR), recombinant E.
coli Trx1, bovine glutathione peroxidase (GPx), insulin,
NADPH, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), APAP, NAPQI,
menadione (2-methyl-1,4-napthoquinone), reduced glutathione (GSH),
oxidized glutathione (GSSG), phosphatase inhibitors (catalog no. P2850,
which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole), and protease inhibitor cocktail (catalog
no. P2714, which contains 4-(2-aminoethyl)benzenesulfonyl fluoride,
E-64, bestatin, leupeptin, aprotinin, and EDTA) were purchased from
Sigma-Aldrich (St. Louis, MO). Sodium aurothiomalate hydrate was from
Aldrich (Milwaukee, WI). Human recombinant TrxR mutant enzyme (Sec498Cys)
was from AbFrontier (Seoul, Korea). Pooled human liver microsomes
(HLM) and recombinant human P450s 1A2, 2E1, and 3A4 were purchased
from BD Genetest (Woburn, MA). N-(Biotinoyl)-N′-(iodoacetyl) ethylenediamine (BIAM) was from Molecular
Probes (Eugene, OR) and horseradish peroxidase (HRP)-conjugated streptavidin
from GE Healthcare (Piscataway, NJ). Enhanced chemiluminescence (ECL)
immunoblot detection reagents were from Thermo Scientific (Rockford,
IL).

Jan Y.H., Heck D.E., Dragomir A.C., Gardner C.R., Laskin D.L, & Laskin J.D. (2014). Acetaminophen Reactive Intermediates Target Hepatic Thioredoxin Reductase. Chemical Research in Toxicology, 27(5), 882-894.

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Recombinant TrxR1 Mutant Enzyme Analysis

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Human recombinant TrxR1 mutant enzyme (Sec498Cys) was from AbFrontier (Seoul, Korea). Dulbecco’s modified Eagle’s medium (DMEM; containing 4500 mg/L D-glucose, 110 mg/mL sodium pyruvate, and 584 mg/L L-glutamine; catalog no. 11995–065), fetal bovine serum, puromycin, and penicillin/streptomycin were from Life Technologies (Grand Island, NY). The SuperSignal West Pico Chemiluminescense Substrate kit was from Thermo Scientific (Rockford, IL). Purified rat cytosolic TrxR, recombinant human Trx1, HN2, insulin, NADPH, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), phosphatase inhibitor cocktail (catalog no. P2850, which contains microcystinLR, cantharidin, and (−)-p-bromotetramisole), protease inhibitor cocktail (catalog no. P2714, which contains 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, bestatin, leupeptin, aprotinin, and EDTA), and all other chemicals were from Sigma (St. Louis, MO).

Jan Y.H., Heck D.E., Casillas R.P., Laskin D.L, & Laskin J.D. (2015). Thioredoxin Cross-Linking by Nitrogen Mustard in Lung Epithelial Cells: Formation of Multimeric Thioredoxin/Thioredoxin Reductase Complexes and Inhibition of Disulfide Reduction. Chemical research in toxicology, 28(11), 2091-2103.

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