Ultra low adherent culture dishes

Manufactured by Corning

Ultra-low adherent culture dishes are designed to promote the suspension growth of cells. They feature a surface treatment that minimizes cell attachment, allowing for the formation of spheroids, organoids, and other 3D cell culture models.

Automatically generated - may contain errors

Lab products found in correlation

Tryple select 1, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

2 protocols using ultra low adherent culture dishes

Differentiation of iPS Cells into Motor Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detailed description can be found in the Supplemental Materialssection. Briefly, iPS cells were maintained on a monolayer of neomycin selected
mouse embryonic fibroblasts (MEFs; Millipore) in hiPS media (Bilican et al., 2012 (link); Dimos et al., 2008 (link)). To generate motor neurons,
undifferentiated iPS cells were incubated with 10 μM Y27632 (Calbiochem)
then passaged, triturated, and placed into ultra-low adherent culture dishes
(Corning) for seeding of embryoid bodies (EBs). For the first 11 days, cells
were kept in suspension in hiPS media without bFGF. At day 11, EBs were switched
to neural induction medium. At day 28, EBs were dissociated, transfected using
Lonza Nucleofector with mRNA beacons and HB9 (9Kb)-promoter-GFP, and plated onto
PDL/laminin-coated (BD Biosciences) glass (Kaech
and Banker, 2006). To prepare the cells for live imaging, cells were
allowed to settle on glass coverslips flipped over primary glial monolayers.

Alami N.H., Smith R.B., Carrasco M.A., Williams L.A., Winborn C.S., Han S.S., Kiskinis E., Winborn B., Freibaum B.D., Kanagaraj A., Clare A.J., Badders N.M., Bilican B., Chaum E., Chandran S., Shaw C.E., Eggan K.C., Maniatis T, & Taylor J.P. (2014). Axonal transport of TDP-43 mRNA granules in neurons is impaired by ALS-causing mutations. Neuron, 81(3), 536-543.

+ Open protocol

+ Expand

Seeding hADSCs on Microcarriers

Check if the same lab product or an alternative is used in the 5 most similar protocols

hADSCs were seeded and incubated at 37 °C in a humidified atmosphere containing 5% CO₂. After cells reached 80% confluence, they were harvested by TryplE™ select (1×) (Gibco). Cells were seeded at the density of 10⁴ cells per mg of each type of MCs in the ultra-low adherent culture dishes (corning). Every two days, 80% percentage of culture medium was aspirated and replaced with an equal volume of fresh medium. To investigate cell attachment and growth, a sample of the MCs was taken at the 1^st, 3^rd, and 7^th day of cell culture and analyzed by fluorescent microscopy after DAPI staining. Accordingly, cells on the surface of the MCs were fixed with paraformaldehyde 4% (Sigma Aldrich) and stained with DAPI (Invitrogen™). Images were taken with a fluorescent microscope (Olympus DP80).

Asadniaye Fardjahromi M., Razmjou A., Vesey G., Ejeian F., Banerjee B., Chandra Mukhopadhyay S, & Ebrahimi Warkiani M. (2020). Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells. RSC Advances, 10(34), 20118-20128.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!