Mixed cellulose ester membrane filter

Manufactured by Cytiva

Sourced in United Kingdom, Germany

Mixed cellulose ester membrane filters are a type of laboratory filtration equipment used to separate and isolate particles, cells, or other materials from liquid samples. These filters are made from a combination of cellulose esters and provide a consistent and reliable filtration performance.

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Lab products found in correlation

Oasis hlb 3 cc 60 mg cartridges, by Waters Corporation (1 mentions) Trisodium citrate dihydrate, by Merck Group (1 mentions) Milli q element, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Scintillation fluid, by Research Products International (1 mentions) Milli q system, by Merck Group (1 mentions) Formic acid, by Mallinckrodt (1 mentions) Formic acid, by Scharlab (1 mentions) Acetonitrile, by Honeywell (1 mentions) Milli q plus system, by Merck Group (1 mentions) Gf a glass microfiber filter, by Cytiva (1 mentions)

6 protocols using mixed cellulose ester membrane filter

SPE-Based Sample Pretreatment for Analyses

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An SPE step was applied prior to analysis to pre-concentrate the sample. All samples were filtered through 0.45 μm mixed cellulose ester membrane filter (Whatman, Dassel, Germany). SPE was performed using Oasis HLB cartridges (60 mg). The cartridges were conditioned by washing and rinsing with 6 mL MeOH and 6 mL Milli-Q water.
The water samples (IWW was four times diluted with MilliQ water, i.e. 25 mL sample in 100 mL; EWW and SW was 100 mL, no dilution) were loaded onto the cartridges, percolated by gravity (flow rate around 3 mL/min) and vacuum dried for approximately 15 min. Analytes were eluted with 5 mL MeOH. The extracts were evaporated to dryness at 35°C under a gentle stream of nitrogen and reconstructed in 1 mL of 10:90 MeOH:H 2 O. Analyses were performed by injecting 20 μL of the final extract into the

Hernández F., Ibáñez M., Botero-Coy A.M., Bade R., Bustos-López M.C., Rincón J., Moncayo A, & Bijlsma L. (2015). LC-QTOF MS screening of more than 1,000 licit and illicit drugs and their metabolites in wastewater and surface waters from the area of Bogotá, Colombia. Analytical and bioanalytical chemistry, 407(21).

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Wastewater Solid Phase Extraction

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Wastewater samples were vacuum-filtered through a glass microfiber filter 1.6 µm GF/A (Whatman, Kent, U.K.) and a 0.45 µm mixed cellulose ester membrane filter (Whatman, Kent, U.K.) before extraction, according to the procedures of each laboratory. SW was not filtered. The method is described in detail elsewhere (Bade et al., 2015c) . Briefly, solid phase extraction (SPE), using OASIS HLB 3 cc/60 mg cartridges (Waters Corp., Milford, MA, USA), was applied to all water samples in order to extract the analytes. Cartridges were conditioned with 6 mL of MeOH and equilibrated with 6 mL of Milli-Q water. Then 100 mL of the samples (IWW was diluted four times with Milli-Q water, i.e. 25 mL sample in 100 mL) were passed through the cartridges by gravity and vacuum-dried for approximately 15 min. The analytes were eluted with 6 mL of MeOH and the extracts were evaporated to dryness at 35°C under a gentle nitrogen stream and finally reconstituted in 1 mL of 10 % MeOH aqueous solution.

Rousis N.I., Bade R., Bijlsma L., Zuccato E., Sancho J.V., Hernandez F, & Castiglioni S. (2017). Monitoring a large number of pesticides and transformation products in water samples from Spain and Italy. Environmental research, 156.

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Biotin-labeled DNA Hybridization Detection

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Trisodium citrate dihydrate, tetrachloroauric(III) acid, sodium hydroxide solutions (10 M in water), and biotin were purchased from Sigma-Aldrich (Italy). biotinylated and unbiotinylated 11-mer oligo deoxyribonucleotide (biotinDNA, 5’-AGCAGCCTAAG-3’-biotin, and DNA, 5’-AGCAGCCTAAG-3’, respectively. T_m = 34.0 °C), successfully employed in previous studies for the detection of non-amplified human genomic DNA [13 (link)], were purchased from Thermo Fisher Scientific, Inc. Streptavidin from Streptomyces avidinii, provided in lyophilized form in 10 mM PBS, pH 7.4, was purchased from Invitrogen (Italy). Mixed cellulose ester membrane filters were purchased from Whatman (UK). Phosphate buffered saline (PBS) solutions at pH 7.4 (137 mM NaCl, 2.7 mM KCl, phosphate buffered 10 mM) were obtained from Amresco (Italy). Ultra-pure water (Milli-Q Element, Millipore) was used for all the experiments.

D'Agata R., Palladino P, & Spoto G. (2017). Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation. Beilstein Journal of Nanotechnology, 8, 1-11.

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Phosphate Uptake Assay in Salmonella

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Wild-type (14028s) or mgtC (EL4) Salmonella were grown in MOPS medium containing 10 μM MgCl₂ and 500 μM K₂HPO₄ during 3 h. Wild-type (14028s) or ∆3Pi (RB39) cells harboring either pVector or pMgtC were grown in MOPS containing 250 μM MgCl₂ and 500 μM K₂HPO₄ until OD₆₀₀ ∼0.2, at which point, MgtC expression was induced for 15 min with the addition of 750 μM IPTG. To assay the transport of Pi, 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K₂H³²PO₄ at a concentration of 2 mM K₂H³²PO₄, PerkinElmer catalog number NEX055) was added to 1 mL cell suspension. At the indicated time points, 50 μL of each sample was submitted to rapid filtration through 0.45 μm mixed cellulose ester membrane filters (Whatman) with an applied vacuum. The filters were washed three times with 1 mL PBS buffer and subsequently soaked in 5 mL scintillation fluid (Research Products International). The amount of radioactivity taken up by the cells was determined with a scintillation counter (Triathler multilabel tester, HIDEX) using the ³²P-window and by counting each vial for 20 s. Radioactive counts per minute were normalized by protein content using a Rapid Gold BCA Protein Assay Kit (Pierce). ³²Pi uptake of each sample was normalized against the corresponding control in each independent experiment.

Bruna R.E., Kendra C.G., Groisman E.A, & Pontes M.H. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proceedings of the National Academy of Sciences of the United States of America, 118(11), e2021370118.

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HPLC-MS Compound Purification

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HPLC-grade methanol (MeOH), and formic acid (N 98% w/w) were purchased from Mallinckrodt Baker (Deventer, The Netherlands). The ultrapure water was obtained by purifying demineralized water in a Milli-Q system from Millipore (Bedford, MA, USA). SPE cartridges used were Oasis HLB 3 mL (60 mg) from Waters (Milford, MA, USA). Polytyrosine-1,3,6 standard used for mass axis calibration was purchased from Cs Bio Co. (Menlo Park, CA, USA). Mixed cellulose ester membrane filters (0.45 μm) were purchased from Whatman (Dassel, Germany).

Bade R., Causanilles A., Emke E., Bijlsma L., Sancho J.V., Hernandez F, & de Voogt P. (2016). Facilitating high resolution mass spectrometry data processing for screening of environmental water samples: An evaluation of two deconvolution tools. The Science of the total environment, 569-570.

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Analytical Standards for Mass Spectrometry

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For information regarding the reference standards used for QTOF and LTQ Orbitrap analysis (see Electronic Supplementary Material (ESM), Section 2.1). HPLC-grade methanol (MeOH), ammonia solution (25%), formic acid (HCOOH, 98-100%) were acquired from Scharlau (Barcelona, Spain) and acetonitrile for LC-MS, from Riedel de Haen (Seelze, Germany). HPLC-grade water was obtained by purifying demineralised water in a Milli-Q plus system from Millipore (Bedford, MA, USA). SPE cartridges used were Oasis HLB 3 mL (60 mg) from Waters (Milford, MA, USA). 0.45 µm mixed cellulose ester membrane filters and 1.6 µm GF/A glass microfiber filters were purchased from Whatman (Kent, UK).

Bade R., Rousis N.I., Bijlsma L., Gracia-Lor E., Castiglioni S., Sancho J.V, & Hernandez F. (2015). Screening of pharmaceuticals and illicit drugs in wastewater and surface waters of Spain and Italy by high resolution mass spectrometry using UHPLC-QTOF MS and LC-LTQ-Orbitrap MS. Analytical and bioanalytical chemistry, 407(30).

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