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Hc pl apo 63x 1.30 glyc corr cs2

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The HC PL APO 63x/1.30 GLYC CORR CS2 is a high-numerical aperture objective lens designed for use in light microscopy applications. It features a 63x magnification and a numerical aperture of 1.30, providing high resolution and light-gathering capabilities. The lens is corrected for chromatic and spherical aberrations, and is compatible with glycerol immersion media.

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Lab products found in correlation

Dmi 6000 deconvolution microscope, by Leica (1 mentions) Huygens professional version 17, by Scientific Volume Imaging (1 mentions)

3 protocols using hc pl apo 63x 1.30 glyc corr cs2

1

Super-Resolution Microscopy Imaging Protocol

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Images were acquired with a vt-instant Structured Illumination Microscope (vt-iSIM; BioVision Technologies) equipped with the 405nm 100mw, 488nm 150Mw, 561nm 150mW, 642nm 100mW and 445nm 75 mW lasers, two ORCA-Fusion sCMOS cameras and the Leica HC PL APO 63x/1.30 GLYC CORR CS2, HC PL APO 63x/1.40 OIL CS2 and HC PL APO 100x/1.47 OIL CORR TIRF objectives. Images were acquired in three dimensions (3D) and afterward deconvolved using Huygens (Scientific Volume Imaging), as described before.90 (link)
Curnutte H.A., Lan X., Sargen M., Ao Ieong S.M., Campbell D., Kim H., Liao Y., Lazar S.B, & Trcek T. (2023). Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila. Cell reports, 42(7), 112723.
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2

Super-Resolution Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were acquired with a vt-instant Structured Illumination Microscope (vt-iSIM; BioVision Technologies) equipped with the 405nm 100mw, 488nm 150Mw, 561nm 150mW, 642nm 100mW and 445nm 75 mW lasers, two ORCA-Fusion sCMOS cameras and the Leica HC PL APO 63x/1.30 GLYC CORR CS2, HC PL APO 63x/1.40 OIL CS2 and HC PL APO 100x/1.47 OIL CORR TIRF objectives. Images were acquired in three dimensions (3D) and afterward deconvolved using Huygens (Scientific Volume Imaging), as described before.90 (link)
Karlin S, & Burge C. (1996). Trinucleotide repeats and long homopeptides in genes and proteins associated with nervous system disease and development.. Proceedings of the National Academy of Sciences of the United States of America, 93(4), 1560-1565.
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3

Multimodal Microscopy Imaging and Deconvolution

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DNA FISH/IF samples were imaged using a Leica DMI 6000
Deconvolution Microscope, with a z-stack collected for each channel (4
μm; step size, 0.2 μm). The objectives used were the
Leica HC PL APO 63x/1.30 GLYC CORR CS2 objective and the Leica HCX PL
APO 100X/1.40- 0.70na OIL objective. Samples were also imaged with a
ZEISS Laser Scanning Microscope (LSM) 800 with the ZEISS i
Plan-Apochromat 63x/1.4 Oil DIC M27 objective, with a z-stack collected
for each channel (step size, 0.37 μm). Deconvolution was
performed using Huygens Professional version 17.04 (Scientific Volume
Imaging, The Netherlands, software available at http://svi.nl) using the built-in theoretical point
spread function, the classic maximum likelihood estimation (CMLE)
algorithm, a signal to noise ratio of 20, and 50 iterations.
Quinodoz S.A., Ollikainen N., Tabak B., Palla A., Schmidt J.M., Detmar E., Lai M.M., Shishkin A.A., Bhat P., Takei Y., Trinh V., Aznauryan E., Russell P., Cheng C., Jovanovic M., Chow A., Cai L., McDonel P., Garber M, & Guttman M. (2018). Higher-order inter-chromosomal hubs shape 3D genome organization in the nucleus. Cell, 174(3), 744-757.e24.
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