MDA-MB-231 and MCF-7 cells were cultured in RPMI-1640 and DMEM/Ham’s F-12 (Gibco Invitrogen, Carlsbad, CA) media, respectively. Both RPMI and DMEM were supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) and 1% penicillin/streptomycin (Hyclone, Logan, UT). Breast cancer cells were grown in a humidified atmosphere (95% air and 5% CO2) until they reached ~80%–90% confluency. Confluent cells were treated with trypsin (0.25% Trypsin-EDTA, Hyclone), harvested by centrifugation, plated on a 6-well plate (~0.5 × 106 cells per well), and treated with AA (sodium salt, 100 μM) (Sigma Chemicals, St. Louis) for 24 h with and without prior treatment with the 5-LOX inhibitor, nordihydroguaiaretic acid (NDGA, 5 μM; Sigma Chemicals), and zileuton (100 μM; Sigma Chemicals). Control and treated cells were collected, washed, and subject to further analyses as described in the text.
Aa sodium salt
Manufactured by Merck Group
Sourced in United States
AA (sodium salt) is a laboratory reagent used in various analytical and research applications. It is the sodium salt form of the chemical compound ascorbic acid, which is commonly known as vitamin C. This product is supplied as a powder and is typically used as a reducing agent, antioxidant, or pH buffer in laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Zileuton, by Merck Group (1 mentions)
Nordihydroguaiaretic acid ndga, by Merck Group (1 mentions)
Dmem ham s f12, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Sequencing grade trypsin, by Promega (1 mentions)
Trifluoroacetic acid tfa, by Thermo Fisher Scientific (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine tcep, by Merck Group (1 mentions)
2 protocols using aa sodium salt
1
Arachidonic Acid Metabolism in Breast Cancer
2
Comprehensive Ginsenoside Extraction and Purification
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The AA sodium salt (AAI 63%, AAII 31%), 10% buffered neutral formalin, calcium chloride, tris(2-carboxyethyl)phosphine (TCEP), ammonium bicarbonate, and guanidine buffer guanidine hydrochloride 8 M were purchased from Sigma (St. Louis, MO, USA). Ginseng Radix Rubra was purchased from the Chinese herbal medicine store (Chang Sheng, Taipei, Taiwan). The ginsenoside Rg1 was purchased from Nacalai Tesque (Kyoto, Japan). Ginsenosides Rb1 and Rd were purchased from Extrasynthèse (Genay, France). HPLC-grade acetonitrile (ACN) was purchased from Merk (Darmstadt, Germany). High performance liquid chromatography (HPLC)-grade isopropanol (IPA) was purchased from Mallinckrodt Baker Inc. (Lexington, KY, USA). Ethylenediaminetetraacetic acid disodium salt (EDTA 2Na) and 3-[(3-cholamidopropyl)dimethylammonio]propansesulfonic acid (CHAPS) were purchased Wako Pure Chemical (Osaka, Japan). Trifluoroacetic acid (TFA) was purchased from Alfa Aesar (Heysham, UK). Sequencing grade trypsin was purchased from Promega (Fitchburg, WI, USA). 4-[2-(Dimethylamino)-ethylaminosulfonyl]-7–chloro -2,1,3-benzoxadiazole (DAABD-Cl) was purchased from Tokyo Chemical Industry Inc. (Tokyo, Japan).
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