Jca bm9030

Serum alanine aminotransferase (ALT) levels in peripheral blood, an indicator of hepatocellular injury, were measured using a standard spectrophotometric method with an automated clinical analyzer (JCA-BM9030; JEOL, Ltd., Tokyo, Japan).

White blood cell count, red blood cell count, hemoglobin, hematocrit, platelet count, total protein, albumin, total bilirubin, AST, ALT, LDH, γ-GTP, ALP, urea nitrogen, uric acid, creatinine, total cholesterol, HDL-Cholesterol, LDL cholesterol, triglyceride, sodium, potassium, chloride, HbA1c, and fasting blood glucose were measured. White blood cells, red blood cells, hemoglobin, hematocrit, and platelets were measured using Sysmex XE-2100 automated hematology analyzer (Sysmex, Kobe, Japan). Total protein, albumin, total bilirubin, AST, ALT, LDH, γ-GTP, ALP, urea nitrogen, uric acid, creatinine, total cholesterol, HDL-cholesterol, LDL cholesterol, triglyceride, sodium, potassium, and chloride were measured with LABOSPECT 008α (Hitachi High-Tech, Tokyo, Japan) and JCA-BM8060 (JEOL Ltd., Tokyo, Japan). HbA1c and fasting blood glucose levels were measured with JCA-BM9030 and JCA-BM9130 (JEOL Ltd., Tokyo, Japan).

A questionnaire was used to assess smoking, alcohol, breakfast and physical activity habits, and history of dyslipidemia, hypertension, and diabetes at each annual examination. Height and weight were measured without shoes or heavy clothing, and body mass index (BMI) was calculated (kg/m²). Blood pressure was measured with the participant in a sitting position. Blood samples were collected after an overnight fast and measurements were made using automatic clinical chemistry analyzers (HITACHI 7250, 7600, and 7700; Hitachi, Tokyo, Japan) for triglyceride, low-density lipoprotein (LDL)-, and high-density lipoprotein (HDL)-cholesterol. Blood glucose and HbA1c levels were determined using an automated analyzer (JCA-BM9030; JEOL, Tokyo, Japan). The HbA1c value was converted to the National Glycohemoglobin Standardization Program equivalent value (%) using the following formula: HbA1c = 1.02 × HbA1c (Japan Diabetes Society) + 0.25.^{18 (link)}