HUVEC cells were purchased from PromoCell (Heidelberg, Germany) and cultured in EBM2 medium containing 2% fetal bovine serum and endothelial cell growth supplement (PromoCell) at 37 °C. Cell growth was observed by inverted microscope. The logarithmic phase endothelial cells were used during the experiment. Rhamnazin (98%, Sigma-Aldrich, St. Louis, MO) was dissolved in DMSO to prepare required concentration.
Rhamnazin
Manufactured by Merck Group
Sourced in United States
Rhamnazin is a laboratory equipment product manufactured by Merck Group. It serves as a specialized tool for scientific research and analysis. The core function of Rhamnazin is to facilitate specific tasks within the laboratory environment, though its precise intended use is not included in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Endothelial cell growth supplement (ecgs), by PromoCell (1 mentions)
Huvec cells, by PromoCell (1 mentions)
Escherichia coli lps, by Merck Group (1 mentions)
Isoquercetin, by Merck Group (1 mentions)
Narirutin, by Merck Group (1 mentions)
Sakuranetin, by Merck Group (1 mentions)
Isovitexin, by Merck Group (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Vitexin, by Merck Group (1 mentions)
Taxifolin, by Merck Group (1 mentions)
Apigenin, by Carl Roth (1 mentions)
Astragalin, by Carl Roth (1 mentions)
Isokaempferide, by Merck Group (1 mentions)
Luteoloside, by Merck Group (1 mentions)
Myricetin, by ChromaDex (1 mentions)
Eriodictyol, by ChromaDex (1 mentions)
Naringenin, by Carl Roth (1 mentions)
Tiliroside, by Carl Roth (1 mentions)
Kaempferitrin, by Merck Group (1 mentions)
Prunetin, by Merck Group (1 mentions)
5 protocols using rhamnazin
1
HUVEC Cell Culture and Rhamnazin Treatment
2
Rhamnazin Alleviates LPS-Induced Lung Injury
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Sprague–Dawley rats (adult males, n=60, weighing 250 to 300 g) were provided by the animal house of Changzhou jintan district people’s hospital. The animals were kept under a 12h by 12h light and dark circadian cycle and the under controlled conditions of temperature and humidity. The animals were fed ad libitum, with the standard rat diet and water. All the animals’ up-keeping procedures were carried out with approval from the institutional ethical committee and in accordance with the Principles of Laboratory Animal Care (NIH publication 85−23, revised in 1985). All efforts were made to minimize the number of animals and their suffering.
Rhamnazin and Escherichia coli LPS were procured from Sigma (USA). The animals were assigned to six groups of 10 rats each at random viz. 1) Control (phosphate buffered saline); 2) LPS group (5 mg/kg, administered through intratracheal instillation); (3) LPS + vehicle; 4) LPS+Rhamnazin (5 mg/kg) group; (5) LPS+ Rhamnazin (10 mg/kg) group; (6) LPS + Rhamnazin (20 mg/kg) group. Saline and Rhamnazin were intra-peritoneally (i.p.) administered 48h before the LPS challenge. The rats were euthanized at 24 hours post-LPS administration. The euthanasia was followed by the collection of lung samples for subsequent experimental analysis.
Rhamnazin and Escherichia coli LPS were procured from Sigma (USA). The animals were assigned to six groups of 10 rats each at random viz. 1) Control (phosphate buffered saline); 2) LPS group (5 mg/kg, administered through intratracheal instillation); (3) LPS + vehicle; 4) LPS+Rhamnazin (5 mg/kg) group; (5) LPS+ Rhamnazin (10 mg/kg) group; (6) LPS + Rhamnazin (20 mg/kg) group. Saline and Rhamnazin were intra-peritoneally (i.p.) administered 48h before the LPS challenge. The rats were euthanized at 24 hours post-LPS administration. The euthanasia was followed by the collection of lung samples for subsequent experimental analysis.
3
Analytical Standards for Phytochemicals
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Analytical standards of apigetrin (apigenin 7-glucoside), chrysin, prunetin, rhamnazin, morin, isoquercetin, vitexin, isovitexin, quercitrin, kaempferitrin, taxifolin, luteolin, 3-O-methylquercetin, kaempferol, hyperoside, rutin, isorhamnetin, isokaempferide, rhamnetin, sakuranetin, luteoloside, eriodictyol-7-O-glucopyranoside, narirutin, naringin, LC grade acetonitryl, DMSO, DPPH radical, ampicillin, bovine serum, ferric chloride, hyaluronidase from bovine testes type I -S, sodium phosphate buffer pH 7, oleanolic acid, 2,4,6-tris(2-pyridyl)-s-triazine TPTZ, and hyaluronic acid were obtained from Sigma-Aldrich Fine Chemicals (St. Louis, MI, USA). Naringenin, astragalin, apigenin, tiliroside, and nicotiflorin were purchased from Roth (Karlsruhe, Germany). Dihydromyricetin was supplied by LGC (LGC Group, Teddington, UK), and laricitrin by Extrasynthese (Lyon, France). Quercetin was obtained from Fluka (Buchs, Switzerland). Catechin, luteolin-7-O-glucoside, luteolin 3,7-diglucoside, Naringenin 7-O-glucoside, eriodictyol, and myricetin were supplied by ChromaDex (Irvine, CA, USA). LC-MS grade water was prepared using a Millipore Direct-Q3 purification system (Bedford, MA, USA). Ethanol was obtained from Avantor Performance Materials (Gliwice, Poland).
4
Phytochemical Screening of Flavonoids
5
Cell Culture and Transfection Protocol
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Lewis lung carcinoma (LLC) cell line and NSCLC cell line H1975 were purchased from American Type Culture Collection (ATCC, Rockville, Maryland, USA) and cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Gibco), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (Gibco) in the presence of 5% CO2 at 37 °C. Rhamnazin (98%, Sigma, Louis, MO, USA) was dissolved in dimethyl sulfoxide (DMSO) to prepare required concentrations. (Shanghai, China). Non-target shRNA control (sh-NC) was used as negative control. For the overexpression of VEGFR2, full-length human VEGFR2 (VEGFR2-OE) was cloned into a pEX-3 vector (GenePharma, Shanghai, China). The empty vector was used as the control. Cellular transfection was performed by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) for 48 h. Subsequently, these cells were collected for western blotting analysis.
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