This investigation was conducted on 9 beagle dogs (Canis familiaris), with an age range from 16 – 36 months. Four sites per animal (maxillary and mandibular canines) were included and allocated to one of the grafting materials. As such, a total of 36 sites were surgically treated, 18 as control and 18 as test. Three animals were sacrificed at each interval (at 2, 6, and 10 weeks postoperatively) providing a total of 12 (6 test and 6 control) sites for evaluation. Approval by the NAMSA Northwood Division Institutional Animal Care and Use Committee (IACUC) was obtained before conducting the study. The sites were predetermined to ensure equal and equivalent distribution of sites and material among the animals included. Soft tissue thickness and coverage, local tissue reaction, and tissue integration were evaluated following the treatment of previously created mucogingival recession defects with two different soft tissue substitutes combined with CAF. Each animal received two porcine-derived materials {test (NovoMatrix™ LifeCell, California, USA) and two control (Mucograft, Geistlich Pharma AG, Wolhusen, Switzerland)}.
Mucograft
Manufactured by Geistlich Pharma
Sourced in Switzerland
Mucograft is a collagen matrix product designed for use in dental and oral surgery procedures. It provides a 3-dimensional scaffold for tissue regeneration. The core function of Mucograft is to facilitate the healing process and support the body's natural regenerative capabilities.
Automatically generated - may contain errors
7 protocols using mucograft
1
Soft Tissue Substitutes in Canine Mucogingival Defects
2
Characterization of Bilayered Porcine Collagen Matrix
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Mucograft® (Geistlich Pharma AG Wolhusen, Suisse) is a CE-marked and FDA-approved three-dimensional matrix registered at the time of this study for intraoral application. It is made from pure collagen obtained by a proprietary standardized manufacturing process and sterilized by gamma irradiation. This bilayered porcine collagen matrix (BPCM) consists of collagen type I and type III of porcine origin without further cross-linking or chemical treatment. The matrix consists of a bilayered structure with a thin, smooth and low-porosity compact layer (CL) in combination with a more porous three-dimensional spongy layer (SL). The CL allows suturing to the mucosal margins. The SL is turned towards the bone defect and/or soft tissue and thereby enables tissue ingrowth to support wound healing.
3
Collagen Matrices in Tissue Engineering
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Two types of collagen matrices were used in this study. Mucoderm (Botiss Biomaterials GmbH, Zossen, Germany) is a 3D collagen matrix, a cell-free structure with a surface layer on the upper side of the matrix and a porous internal structure with a parallel arrangement of densely packed bundles consisting of highly purified porcine collagen type I and III without cross-links and elastin.
Mucograft (Geistlich Pharma AG, Wolhusen, Switzerland) is a 3D collagen matrix consisting of highly purified porcine collagen type I and III without cross-links, containing two layers of dense collagen and a spongy scaffold [15 (link),16 (link),17 (link)].
Mucograft (Geistlich Pharma AG, Wolhusen, Switzerland) is a 3D collagen matrix consisting of highly purified porcine collagen type I and III without cross-links, containing two layers of dense collagen and a spongy scaffold [15 (link),16 (link),17 (link)].
4
Protein Release from Biomaterial Scaffolds
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Folded PCL3%-E membranes (n = 2) and Mucograft® (Geistlich Pharma) (n = 1), with an area of 1 cm2 were dried at 40 °C for 12 h before being weighed pre- (weightdry) and post-soaking (weightsoaked) in 1 mL of 100% PL (Cook Regentec) for 15 min at RT to mimic the clinical setting [10 (link),12 (link)]. The weight increase (%) was calculated as an indicator of absorption capacity and normalised to the sample area. PL soaked PCL3%-E (n = 2) and Mucograft® were incubated in simulated body fluid (SBF) at 37 °C, and fluid samples (60 μL) were collected sacrificially over time replaced with an equal volume of SBF. A bicinchoninic acid (BCA) assay was carried out, following the manufacturers protocol, to quantify protein concentration (μg/mL) and due to the sacrificial nature of the release assay, a dilution factor was also accounted for in each sample.
5
Comparative Analysis of Collagen Membranes
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Five different collagen membranes; Bio-Gide® (BG), Fibro-Gide® (FG), Mucograft® (MG, all Geistlich Pharma AG, Wolhusen, Switzerland), Collagen Membrane-P (CMP; Genoss Co. Ltd.; Gwanggyo-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea), Collagen Graft 2 (CG2; Genoss Co. Ltd.) were cut into rectangular pieces 15 mm × 10 mm. The membranes were then placed into 1 mL of serum-free DMEM (Sigma, St. Louis, MO, USA) for 72 h in a humidified atmosphere at 37 °C without shaking. Supernatants were collected with a pipette, and cells were immediately exposed to the undiluted solution. The other samples of collagen membranes were digested with 0.1% collagenase type 1 (Invitrogen Corporation, Grand Island, NY, USA) at room temperature with continuous shaking overnight followed by heating at 72 °C for 30 min. After centrifugation at 15,000 g for 2 min, the lysates were prepared to expose to cells immediately.
6
Comparing Root Coverage Surgical Protocols
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This study was designed as a preclinical split-mouth randomized controlled pilot study comparing four root coverage surgical protocols: (1) CAF plus a xenogeneic CMX (Mucograft®, Geistlich Pharma AG, Wolhusen, Switzerland) seeded with autologous GMSCs (test group; CMX+GMSCs), (2) the CAF plus a CMX seeded with autologous fibroblasts (control group 1; CMX+FBs), (3) CAF plus a CMX (control 2; CMX) and (4) CAF alone (control 3; CAF). Each animal provided eight study sites, four in the maxilla and four in the mandible (upper second incisors, upper canines, lower canines and the mesial roots of lower first molars) and two healing times (2 and 6 weeks). Each site was randomly assigned to one intervention and healing time using a computer-generated randomization list stratified by hemi-mandible/maxilla, tooth and healing time (IBM SPSS Statistics® v20. JM. Domenech). Allocation to the tested interventions was concealed by using sealed envelopes, which were opened during the surgical procedure once the flaps were raised.
7
Porcine Collagen Matrices for Wound Healing
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Mucograft®(Geistlich Pharma AG, Wolhusen, Switzerland) is a pure collagen
type I and III matrix of porcine origin without further cross-linking. One part of
the matrix is a thin, smooth and low-porosity compact layer (CL) while the other part
is a thicker porous and three-dimensional spongy layer (SL). While the CL has elastic
properties and is supposed to permit suturing to the host mucosal margins, the SL
should enable tissue adherence and mediate wound healing and cell integration.
BioGide®(Geistlich Pharma AG, Wolhusen, Switzerland) is a pure collagen
type I and III membrane. The collagen membrane is processed into a bilayered
structure; one side of the structure is compact and smooth, constituting a
low-porosity compact layer (CL), whereas the other side is a more porous,
three-dimensional spongy layer (SL).
type I and III matrix of porcine origin without further cross-linking. One part of
the matrix is a thin, smooth and low-porosity compact layer (CL) while the other part
is a thicker porous and three-dimensional spongy layer (SL). While the CL has elastic
properties and is supposed to permit suturing to the host mucosal margins, the SL
should enable tissue adherence and mediate wound healing and cell integration.
BioGide®(Geistlich Pharma AG, Wolhusen, Switzerland) is a pure collagen
type I and III membrane. The collagen membrane is processed into a bilayered
structure; one side of the structure is compact and smooth, constituting a
low-porosity compact layer (CL), whereas the other side is a more porous,
three-dimensional spongy layer (SL).
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