Agilent 1290 infinity lc ultra high pressure liquid chromatograph

Manufactured by Agilent Technologies

The Agilent 1290 Infinity LC is an ultra-high-pressure liquid chromatograph capable of operating at pressures up to 1300 bar. It is designed for rapid, high-resolution separation of complex samples.

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Lab products found in correlation

Ab triple tof 5600 6600 mass spectrometer, by AB Sciex (2 mentions) Acquity uplc beh amide column, by Waters Corporation (1 mentions) Low temperature high speed centrifuge, by Eppendorf (1 mentions) Tripletof 6600 mass spectrometer, by AB Sciex (1 mentions)

4 protocols using agilent 1290 infinity lc ultra high pressure liquid chromatograph

Fecal Metabolomics of Offspring

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To uncover the differences in metabolites, fecal samples from 6-week-old offspring in the two groups were subjected to non-targeted metabolomics analysis, which was based on the ultra-high-performance liquid chromatography (UHPLC) (Shanghai Personal Biotechnology).
Liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis was used to identify and quantify the metabolites. The samples were separated using an Agilent 1290 Infinity LC Ultra-High-Pressure Liquid Chromatograph (Agilent, Waters Acquity UPLC BEH amide column, 1.7 μm, 2.1 mm × 100 mm). The quadrupole time-of-flight (QTOF) mass spectrometry conditions were as follows: electrospray ionization (ESI) positive and negative ion modes for detection.
The data were uploaded and stored in the SRA(Sequence Read Archive) of NCBI(national center for biotechnology information) repository [SRP319184].

Yang X., Tang T., Li M., Chen J., Li T., Dai Y, & Cheng Q. (2022). Vascular Endothelial Growth Factor May Be Involved in the Behavioral Changes of Progeny Rats after Exposure to Ceftriaxone Sodium during Pregnancy. Journal of Microbiology and Biotechnology, 32(6), 699-708.

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Metabolomic Analysis of Mysterious Fruit

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SD was purchased from Jiangmen, Guangdong Province, China, and was identified as a plant of the genus mysterious fruit of the Solanaceae by a researcher from Inner Mongolia Medical University. The SD samples are stored at −80°C. Methanol, acetonitrile, and formic acid used in this part of the experiment were chromatographic pure, and the other reagents were analytically pure. AB Triple TOF 5600/6600 Mass Spectrometer (AB SCIEX), Agilent 1290 Infinity LC Ultra-High Pressure Liquid Chromatograph (Agilent), and Low Temperature High Speed Centrifuge (Eppendorf 5430R) were used. Column: Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 mm × 100 mm column; Waters, ACQUITY UPLC HSS T3 1.8 μm, 2.1 × 100 mm column.

Chen Q., Liu T., Bai T., Zhang M., Hu Y., Li J, & Chang F. (2022). Mechanism of Synsepalum dulcificum Daniell. Inhibiting Lung Adenocarcinoma. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 5242179.

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Comprehensive UHPLC-Q-TOF MS Metabolomics

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This experiment uses UHPLC-Q-TOF MS technology combined with data-dependent acquisition methods to perform full-spectrum analysis of samples. AB Triple TOF 5600/6600 mass spectrometer (AB SCIEX) and Agilent 1290 Infinity LC Ultra High-Pressure Liquid Chromatograph (Agilent) were used to obtain primary and secondary mass spectrometry data at the same time and then used XCMS to perform peak extraction and metabolite identification on the data. Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 mm × 100 mm column and HSS T3 1.8 μm, 2.1 mm × 100 mm column were used as the columns.

Xu W., Hu X., Anwaier A., Wang J., Liu W., Tian X., Zhu W., Ma C., Wan F., Shi G., Qu Y.Y., Zhang H, & Ye D. (2021). Fatty Acid Synthase Correlates With Prognosis-Related Abdominal Adipose Distribution and Metabolic Disorders of Clear Cell Renal Cell Carcinoma. Frontiers in Molecular Biosciences, 7, 610229.

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Metabolomic Profiling of Antibiotic-Exposed Offspring

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To determine the differences in metabolites between offspring exposed to antibiotics during pregnancy and Control, we collected the feces of the two groups at 6 weeks old and performed non-targeted metabolomics analysis based on ultra-high performance liquid chromatography (UHPLC), which was carried out by Shanghai Personal Biotechnology.
After metabolite extraction, we used liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis to identify and quantify the metabolites. The samples were separated using an Agilent 1290 Infinity LC Ultra High Pressure Liquid Chromatograph (Agilent)(Waters, ACQUITY UPLC BEH Amide 1.7 μm, 2.1 mm × 100 mm column). The quadrupole time of flight (QTOF) mass spectrometry conditions comprised: Electrospray ionization (ESI) positive and negative ion modes for detection, separation of the samples using UHPLC, and mass spectrometry performed using a Triple TOF 6600 mass spectrometer (AB SCIEX, Framingham, MA USA).
Data processing: The raw data was converted to the .mzXML format using ProteoWizard, and then peak aligned using the XCMS program. Retention time correction and extraction of the peak area were performed. Metabolite structure identification using accurate mass matching (< 25 ppm) and secondary, The method of spectral matching comprised retrieving the self-built database of the laboratory.

Yang X., Tang T., Wen J., Li M., Chen J., Li T., Dai Y, & Cheng Q. (2021). Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy. BMC Microbiology, 21, 166.

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