A competitive ELISA kit (Cell Biolabs, San Diego, CA, United States) was used to measure malondialdehyde (MDA). ELISA immunoassay for MDA-protein adduct products was used for this assay. Moreover, total capacity of antioxidants was measured Capacity Assay Kit (Cell Biolabs) based on copper (II) being reduced to copper (I) by antioxidants. By reducing copper (I) ions, a coupling chromogen increases 490 nm absorbance. The net absorbance was compared to that of the standard curve based on known uric acid concentrations. An ELISA kit for mice catalase (CAT) (Cusabio, Wuhan, China) was used to measure catalase activity.
Competitive elisa kit
Manufactured by Cell Biolabs
Sourced in United States
The Competitive ELISA kit is a laboratory tool designed to detect and quantify the presence of specific analytes in a sample. It utilizes the principle of competitive binding to measure the target analyte concentration. The kit provides the necessary reagents and protocols to perform the assay.
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Lab products found in correlation
4 protocols using competitive elisa kit
1
MDA, Antioxidant, and Catalase Assays
2
Plasma Nitrotyrosine Levels in COVID-19
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Quantitative determination of nitrotyrosine in the plasma of control subjects and COVID-19 patients was performed by a competitive ELISA kit (Cell biolabs, Inc.) as per manufacturer’s instructions.
3
Serum 4HNE-PAs Quantification
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4HNE-PAs level in the serum was determined using competitive ELISA kit from Cell Biolabs, Inc. (STA-838; San Diego, CA, USA), according to the manufacturer’s instructions.
4
Serum 4-HNE Measurement in Rats
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Blood was collected from rat tail vein (n = 3 per group), placed in a centrifuge tube, and centrifuged at 5000 rpm for 10 minutes. The supernatant serum was stored in −80°C until further use. The serum samples were used to measure 4‐HNE by a competitive ELISA kit (Cell Biolabs). The animals were anesthetized using 2% to 3% isoflurane and blood was collected just before the surgery, at 1 week, and 2 weeks (just before euthanizing the animals).
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