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Cd 1 animals

Manufactured by Charles River Laboratories
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CD-1 animals are outbred, general-purpose mice commonly used in research. They are bred to maintain genetic diversity and can be used for a variety of applications.

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Lab products found in correlation

Thy1 yfp h mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Dorsomorphin, by Bio-Techne (1 mentions) Su5402, by Bio-Techne (1 mentions)

4 protocols using cd 1 animals

1

Retinal Cell Characterization in Mice

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All mice were used in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and with the approval of the University of Colorado Denver Institutional Animal Care and Use Committee. Wild-type CD-1 mice were used for retinal sections at embryonic and postnatal stages. Thy1-YFP-H mice were acquired from Jackson Laboratories (stock #3782; Bar Harbor, ME, USA)62 (link) and maintained by outcrossing to CD-1 animals (Charles River Laboratories, Wilmington, MA, USA). Flatmount stains were done with CD-1 mice or the wild-type littermates of Thy1-YFP-H mice. The Prdm16 staining pattern in retinal flatmounts (below) was equivalent in C57BL/6J mice (Jackson Laboratories, stock #664) and at all ages examined (3–25 weeks; data not shown).
Groman-Lupa S., Adewumi J., Park K.U, & Brzezinski JA I.V. (2017). The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina. Investigative Ophthalmology & Visual Science, 58(12), 5421-5433.
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2

Transgenic Mouse Generation for Germ Cell Studies

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C57BL/6J animals bearing the DazlTm1hgu allele were rederived at the Case Transgenic and Targeting Facility, and bred with animals bearing the Stra8-iCre or IRG transgenes. Mixed background (CD1xC57BL/6J) Stra8-iCre2+; DazlTm1hgu/+ males and IRG+; DazlTm1hgu/+ females were crossed to generate Dazl WT and KO offspring with GFP+ male germ cells. Day of birth was considered P0. For all procedures, animals were anesthetized by isoflourane inhalation and death confirmed by decapitation or cervical dislocation. HITS-CLIP, iCLIP, adult testis RNA-Seq, and adult testis Poly-Seq were performed using CD-1 animals purchased from Charles River labs. Spermatogonia PolyA-Seq libraries were generated from FACS-isolated cells from 8 week Stra8-iCre+; IRG+ C57BL/6J males as previously described (Zagore et al., 2015 (link)). All animal procedures were approved by the Institutional Animal Care and Use Committee at CWRU.
Zagore L.L., Sweet T.J., Hannigan M.M., Weyn-Vanhentenryck S.M., Jobava R., Hatzoglou M., Zhang C, & Licatalosi D.D. (2018). DAZL Regulates Germ Cell Survival through a Network of PolyA-Proximal mRNA Interactions. Cell reports, 25(5), 1225-1240.e6.
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3

Embryo Culture with Small Molecule Inhibitors

Cited 2 times
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All animal studies were approved by the Institutional Animal Care and Use Committee, University of Massachusetts, Amherst (protocol # 2015-0042). Embryo culture was performed as described (3 (link)). CD-1 animals (Charles River) were used to generate embryos. The small molecules used include 5 μM Dorsomorphin (Tocris), 12.5 μM DMH1 (Calbiochem), and 40 μM SU5402 (Tocris). DMH1 is capable of inhibiting signaling from ALK2 and ALK3 (13 (link)) whereas Dorsomorphin can inhibit signaling from ALK2, ALK3 and ALK6 (14 (link)). Each drug was dissolved in DMSO and control embryos of similar somite stages were provided with an equal volume of DMSO.
Palaria A., Angelo J.R., Guertin T., Mager J, & Tremblay K.D. (2018). Patterning of the hepato-pancreatobiliary boundary by BMP reveals heterogeneity within the murine liver bud. Hepatology (Baltimore, Md.), 68(1), 274-288.
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4

Transgenic Mouse Generation for Germ Cell Studies

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C57BL/6J animals bearing the DazlTm1hgu allele were rederived at the Case Transgenic and Targeting Facility, and bred with animals bearing the Stra8-iCre or IRG transgenes. Mixed background (CD1xC57BL/6J) Stra8-iCre2+; DazlTm1hgu/+ males and IRG+; DazlTm1hgu/+ females were crossed to generate Dazl WT and KO offspring with GFP+ male germ cells. Day of birth was considered P0. For all procedures, animals were anesthetized by isoflourane inhalation and death confirmed by decapitation or cervical dislocation. HITS-CLIP, iCLIP, adult testis RNA-Seq, and adult testis Poly-Seq were performed using CD-1 animals purchased from Charles River labs. Spermatogonia PolyA-Seq libraries were generated from FACS-isolated cells from 8 week Stra8-iCre+; IRG+ C57BL/6J males as previously described (Zagore et al., 2015 (link)). All animal procedures were approved by the Institutional Animal Care and Use Committee at CWRU.
Zagore L.L., Sweet T.J., Hannigan M.M., Weyn-Vanhentenryck S.M., Jobava R., Hatzoglou M., Zhang C, & Licatalosi D.D. (2018). DAZL Regulates Germ Cell Survival through a Network of PolyA-Proximal mRNA Interactions. Cell reports, 25(5), 1225-1240.e6.
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