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Ephb2

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EPHB2 is a protein that functions as a receptor tyrosine kinase. It is involved in various cellular processes, including cell-cell communication, cell migration, and tissue patterning during development.

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Lab products found in correlation

Cd206, by Cell Signaling Technology (1 mentions) Nis freeware 2, by Nikon (1 mentions) Okadaic acid, by Merck Group (1 mentions) 17 dmag, by Selleck Chemicals (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) β tubulin, by Santa Cruz Biotechnology (1 mentions) Pdgfrα, by Santa Cruz Biotechnology (1 mentions) Multi gauge v3, by Fujifilm (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions) Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Ephb4, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

EphB2 siRNA (2 citations) CRISPR activation plasmid specific for EphB2 (1 citations)

4 protocols using ephb2

1

Immunohistochemical Staining of EPHB2 and CD206

Cited 1 time
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IF assay was implemented according to the methods described previously (26 (link)). The primary antibodies included EPHB2 (1:100, 2D12C6, Santa Cruz Biotechnology) and CD206 (1:100,24595, Cell Signaling Technology).
Liu J., Shen H., Gu W., Zheng H., Wang Y., Ma G, & Du J. (2022). Prediction of prognosis, immunogenicity and efficacy of immunotherapy based on glutamine metabolism in lung adenocarcinoma. Frontiers in Immunology, 13, 960738.
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2

Histopathological and Immunohistochemical Analysis of Mouse Adenocarcinoma

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Part of the tumor masses and normal colon mucosae were analyzed according to standard histochemical procedures. Mouse adenocarcinoma were diagnosed according to the histopathological criteria described by Boivin et al. (22 (link)).
Immunohistochemistry was performed on 4-µm-thick FFPE tissue sections after antigen retrieval with sodium citrate buffer. Goat anti-mouse Krt20 and Lgr5, rabbit anti-mouse EphA2 and EphB2 (Santa Cruz Biotechnology, Santa Cruz, CA, 1:50) were used. The immunostained slides were observed under a microscope, and the image data were analysed using NIS FreeWare 2.10 software (Nikon, Japan).
De Robertis M., Loiacono L., Fusilli C., Poeta M.L., Mazza T., Sanchez M., Marchionni L., Signori E., Lamorte G., Vescovi A.L., Garcia-Foncillas J, & Fazio V.M. (2016). Dysregulation of EGFR pathway in EphA2 cell subpopulation significantly associates with poor prognosis in colorectal cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(1), 159-170.
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3

BCR-ABL Signaling Pathway Modulation

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Imatinib mesylate and ponatinib hydrochloride were obtained from NCE Biomedical Co., Ltd. ApoE-mimetic peptide COG112 (acetyl-LRVRLASHLRKLRKRLL-amide) was synthesized by ChinaPeptides Co., Ltd and purified to > 95% purity. Antibodies to BCR-ABL (b2a2 Junction Specific) (#3908), p-Abl (Tyr245) (#2861) and PP2Ac (#2038) were from CST; antibodies to p-PP2Ac (Y307) (#sc-12615-R), EphB2 (#sc-1763), PDGFRα (#sc-338), β-actin (#sc-47778) and β-tubulin (#sc-9104) were from Santa Cruz; antibodies to SET (#ab1183) was from Abcam. 17-DMAG was acquired from Selleck Chemicals. Okadaic acid (OA) and other reagents and solvents were analytical grade and from Sigma-Aldrich unless noted otherwise.
Wang S., Xie W., Wang D., Peng Z., Zheng Y., Liu N., Dai W., Wang Y., Wang Z., Yang Y, & Chen Y. (2015). Discovery of a small molecule targeting SET-PP2A interaction to overcome BCR-ABLT315I mutation of chronic myeloid leukemia. Oncotarget, 6(14), 12128-12140.
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4

Analyzing EPHB2, EPHB4, and EPZH2 in Cell Lines

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Whole cell extracts of cell lines were used in Western blots for EPHB2 (Santa Cruz, Dallas, TX, USA, #sc-130752), EPHB4 (Santa Cruz, #sc-5536), and GAPDH (Santa Cruz, #sc-51631). Nuclear extracts for Co-IP were prepared and diluted using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, #78835) and RIPA buffer (Life Technologies, Grand Island, NY, USA, 89900). The IP was incubated with JMJD3 antibody (Abcam, #ab38113), Notch intracellular domain (NICD) antibody (Abcam, # ab8925), or EZH2 antibody (Abcam, #ab186006) overnight at 4°C, the protein G Dyna-beads (Life Technologies, 10009D) were added and incubated at 4°C for 3 hours. After washing with RIPA buffer three times, the beads were resuspended in reducing SDS gel loading buffer. The gray-scale assay for each blots was analyzed using MultiGauge V3.0 software (Fujifilm, Tokyo, Japan), and number under each blots presents the relative level compared with the vector or Scrss.
Lian H., Jia X., Shi N., Xie S., Wang J., Wang W., Ma F., Liu H., Wang A., Cheng X, & Liu C. (2018). Notch signaling promotes serrated neoplasia pathway in colorectal cancer through epigenetic modification of EPHB2 and EPHB4. Cancer Management and Research, 10, 6129-6141.
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